补肾健骨胶囊中莫诺苷与马钱苷含量测定  

Determination of Morroniside and Loganin in Bushen Jiangu Capsules

作　　者：明凯利 王海丁 张馨之 田郅扬 黄志军 向阳[1] 陈鹏 曾庆恢 Ming Kaili;Wang Haiding;Zhang Xinzhi;Tian Zhiyang;Huang Zhijun;Xiang Yang;Chen Peng;Zeng Qinghui(Jianmin Pharmaceutical Group Co.Ltd.,Wuhan 430052,China;Aix-Marseille School of Wuhan University of Technology)

机构地区：[1]武汉市健民药业集团,武汉430052 [2]武汉理工大学艾克斯马赛学院

出　　处：《中国药师》2022年第3期534-537,共4页China Pharmacist

摘　　要：目的:建立一种同时检测补肾健骨胶囊中莫诺苷和马钱苷含量的方法。方法:采用Tnature C_(18) (250 mm×4.6 mm, 5μm)色谱柱,柱温30℃,以乙腈-0.8%磷酸溶液为流动相,梯度洗脱,体积流量1.0 ml·min^(-1),检测波长240 nm。结果:莫诺苷在82.010 8~1 640.216 0 ng、马钱苷在63.756 0~1 275.120 0 ng范围内线性关系良好;莫诺苷的平均回收率为94.38%,RSD为2.06%,马钱苷的平均回收率为94.90%,RSD为1.88%(n=6)。结论:该方法操作简便,重复性好,可准确检测补肾健骨胶囊中莫诺苷和马钱苷的含量。Objective: To establish a method for the simultaneous determination of morroniside and loganin in Bushen Jiangu capsules. Methods: The detection was carried out on a Tnature C_(18) (250 mm×4.6 mm, 5 μm) column with the column temperature at 30℃. The mobile phase consisted of acetonitrile and 0.8% phosphoric acid solution with gradient elution. The flow rate was 1.0 ml·min^(-1) and the detection wavelength was 240 nm. Results: The linear relationships of morroniside and loganin were good within the ranges of 82.010 8-1 640.216 0 ng and 63.756 0-1 275.120 0 ng, respectively. The average recovery of morroniside was 94.38%(RSD=2.06%), and that of loganin was 94.90%(RSD=1.88%,n=6). Conclusion: The method is simple and reproducible, and can be used to accurately determine the contents of morroniside and loganin in Bushen Jiangu capsules.

关 键 词：补肾健骨胶囊 莫诺苷 马钱苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]