NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis  

作　　者：CHEN Zhi LIANG Yu-sheng ZONG Wei-cheng GUO Jia-he ZHOU Jing-peng MAO Yong-jiang JI De-jun JIAO Pei-xin Juan J LOOR YANG Zhang-ping 

机构地区：[1]College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,P.R.China [2]Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education,Yangzhou University,Yangzhou 225009,P.R.China [3]Mammalian Nutrition Physiology Genomics,Department of Animal Sciences and Division of Nutritional Sciences,University of Illinois,Urbana,IL 61801,USA [4]College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,P.R.China [5]College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第4期1161-1176,共16页农业科学学报（英文版）

基　　金：supported by the Independent Innovation in Jiangsu Province of China(CX(21)3119);the National Natural Science Foundation of China(31802035,31702095 and 31872324);the Jiangsu Natural Science Fund(BK20181221);Yangzhou Liangde Antibody Bio Tech.,China。

摘　　要：Excess ammonia(NH_(3))in the circulation of dairy animals can reduce animal health and the quality of products for human consumption.To develop effective prevention and treatment methods,it is essential to examine the molecular mechanisms through which excess NH_(3) may affect the mammary gland.The present study used bovine mammary epithelial cells(BMECs)to evaluate the effects of exogenous NH_(4)Cl on the abundance of circular RNAs(circRNAs)using high-throughput sequencing.Among the identified circRNAs,circ02771 was the most significantly upregulated by exogenous NH_(4)Cl(P<0.05),with a fold change of 4.12.The results of the apoptosis and proliferation assays,transmission electron microscopy,H&E staining,and immunohistochemistry revealed that circ02771 increased apoptosis and inflammation.A double luciferase reporter assay revealed that circ02771 targeted miR-194b,and the overexpression of circ02771(pcDNA-circ02771)reduced(P<0.05)the expression of miR-194b and led to apoptosis and inflammation.Circ02771 also enhanced the expression of transforming growth factor beta-induced factor homeobox 1(TGIF1),which is a target gene of miR-194b.Overall,this study suggests that the circ02771/miR-194b/TGIF1 axis plays a role in mediating the effects of NH_(4)Cl on BMECs.Therefore,this axis provides a novel target to help control hazards within the mammary gland from high circulating NH_(4)Cl levels.

关 键 词：NH4CL circ02771 miR-194b TGIF1 bovine mammary epithelial cells 

分 类 号：S858.23[农业科学—临床兽医学]