Determination of inulin-type fructo-oligosaccharides in inulin by HPLC-ELSD  

HPLC-ELSD法测定菊粉中菊粉型果寡糖的含量

作　　者：Xiaoyu Cui Pan Wang Yidian Mo Xuyang Ding Shizhong Chen Hongzhu Guo Qin Hu Zhongjun Li 崔晓雨;王攀;莫伊点;丁旭阳;陈世忠;郭洪祝;胡琴;李中军(北京大学药学院天然药物及仿生药物国家重点实验室,北京100191;北京市药检所(北京市保健食品化妆品检验中心),北京102206)

机构地区：[1]State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China [2]Beijing Institute for Drug Control(Beijing Center for Health Food and Cosmetics Control),Beijing 102206,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2022年第1期23-30,共8页中国药学（英文版）

基　　金：National Natural Science Foundation of China(Grant No.21977005);the Beijing Natural Science Foundation(Grant No.7192101)。

摘　　要：To develop an HPLC-ELSD method for the determination of nine inulin-type fructo-oligosaccharides in inulin,the HPLC-ELSD system consisted of Waters XBridge■ Amide column(4.6 mm×250 mm,5μm)with a gradient elution mobile phase consisting of acetonitrile and water at a flow rate of 1.2 mL/min at 30°C.The detector was an Agilent Technologies 380-ELSD.The drift tube temperature for the ELSD was set at 55°C with a nitrogen flow rate of 1.8 L/min.The injection volume was 15μL.The results showed that the detection range for the nine inulin-type fructo-oligosaccharides was 3.81–30.60μg R^(2)=0.99969 for kestose,3.73–29.97μg R^(2)=0.99981 for nystose,3.82–30.69μg R^(2)=0.99993 for fructosylnystose,3.80–30.48μg R^(2)=0.99995 for GF5,3.73–29.96μg R^(2)=0.99993 for GF6,3.78–30.30μg R^(2)=0.99983 for GF7,3.82–30μg R^(2)=0.99989 for GF8,3.71–29.80μg R^(2)=0.99974 for GF9,3.61–29.00μg R^(2)=0.99970 for GF10,respectively.The recovery of the nine oligosaccharides ranged between 96.48%–100.84%(n=6).The method was simple,accurate,and reproducible that it could be used as an analytical method for evaluating the quality of inulin effectively.建立HPLC-ELSD法测定菊粉中9种蔗果寡糖的含量,色谱系统组成:色谱柱:Waters XBridge■Amide(4.6 mm×250 mm,5μm);流动相:CH_(3)CN–H_(2)O,梯度洗脱;流速:1.2 mL/min;柱温:30°C;检测器:Agilent Technologies 380-ELSD,漂移管温度:55°C,N_(2)流速:1.8 L/min,进样量:1.5μL。实验结果是9种菊粉型果寡糖分别在:蔗果三糖3.81-30.60μg,R^(2)=0.99969;耐斯糖3.73–29.97μg,R^(2)=0.99981;蔗果五糖3.82–30.69μg,R^(2)=0.99993;蔗果六糖3.80–30.48μg,R^(2)=0.99995;蔗果七糖3.73–29.96μg,R^(2)=0.99993;蔗果八糖3.78–30.30μg,R^(2)=0.99983;蔗果九糖3.82–30μg,R^(2)=0.99989;蔗果十糖3.71–29.80μg,R^(2)=0.99974;蔗果十一糖3.61–29.00μg,R^(2)=0.99970范围内线性关系良好,回收率在96.48%–100.84%(n=6)。该方法简单、准确,具有良好的重复性,为有效评价菊粉的质量提供了分析方法。

关 键 词：INULIN Inulin-type fructo-oligosaccharides Quantitative determination 

分 类 号：R917[医药卫生—药物分析学]