基于免疫性PTR的血小板膜糖蛋白及T/B淋巴细胞表达水平研究  

Expression of Platelet Membrane Glycoprotein and T/B Lymphocyte Based on Immune PTR

作　　者：梁静[1] 凯赛尔江·多来提 刘雯[1] 范娜[1] 王瑶[1] 颜国君[3] LIANG Jing;KAIsaierjiang·duolaiti;LIU Wen;FAN Na;WANG Yao;YAN Guo-jun(The sixth affiliated hospital ofxinjiang medical university,Urumqi,Xinjiang,830002,China;Human anatomy department of preclinical college ofxinjiangmedical university,Urumqi,Xinjiang,830011,China;The first division hospital ofxinjiangproduction and constructioncrops,Alar,Xinjiang,843008,China)

机构地区：[1]新疆医科大学第六附属医院,新疆乌鲁木齐830002 [2]新疆医科大学基础医学院人体解剖学教研室,新疆乌鲁木齐830011 [3]新疆生产建设兵团第一师医院医共体一团分院,新疆阿拉尔843008

出　　处：《现代生物医学进展》2022年第4期727-730,736,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81760036)。

摘　　要：目的:探究血小板膜糖蛋白及T/B淋巴细胞免疫在免疫性血小板输注无效发生机制中的重要作用。方法:采用血小板特异性抗体检测试剂盒及流式细胞技术检测免疫性PTR患者血小板特异性抗体(抗GPⅡb/Ⅲa、抗GPIa/Ⅱa、抗GPIb/Ⅸ、抗GPⅣ)、血小板膜糖蛋白(CD36、CD61、CD41a)表达情况及外周血T/B淋巴细胞数量,运用SPSS19.0软件及R软件分析免疫性PTR与血小板膜糖蛋白、外周血T/B淋巴细胞的相关性。结果:免疫性PTR与血小板输注有效者比较,血小板特异性抗体的产生无显著差异,血小板膜糖蛋白CD36和CD61的表达具有显著差异,CD36对免疫性PTR发生风险具有极大的预测价值,外周血CD8^(+)T细胞比例增高,而CD4^(+)T细胞比例减低。结论:免疫性PTR患者产生血小板抗体具体机制不明确,了解患者细胞免疫状态有助于明确免疫性PTR的发生机制,为患者提供更优质的诊疗策略。Objective: To explore the important role of platelet membrane glycoprotein and T cell immunity in the pathogenesis of immune platelet transfusion inefficacy. Methods: Platelet specific antibody(anti gpiib/iiia, anti gpia/iia, anti gpib/ix, anti GPⅣ), platelet membrane glycoprotein(CD36, CD61, CD41a) and t/b lymphocytoplasma in peripheral blood were detected by using platelet specific antibody detection kit and flow cytometry. The expression of immune PTR and platelet membrane glycoprotein were analyzed by spss19.0 software and R software The correlation of t/b lymphocytes in peripheral blood. Results: The expression of platelet membrane glycoprotein CD36 and CD61 were significantly different. CD36 has great predictive value for the risk of immune PTR. The proportion of CD8^(+)T cells in peripheral blood increased, while the proportion of CD4^(+)T cells decreased. Conclusion: The specific mechanism of platelet antibody production in patients with immune PTR is not clear. Understanding the cellular immune status of patients will help to clarify the mechanism of immune PTR and provide better diagnosis and treatment strategies for patients.

关 键 词：免疫性血小板输注无效 血小板膜糖蛋白 血小板特异性抗体 T/B淋巴细胞 

分 类 号：R457[医药卫生—治疗学]