基于近场光纤探针的活细胞无损测温方法研究  

Nondestructive Temperature Measurement Method for Living Cells Based on Near-Field Optical Fiber Probe

作　　者：李韶颖 陈娜 刘真民 陈振宜 刘书朋 商娅娜 王廷云 Li Shaoying;Chen Na;Liu Zhenmin;Chen Zhenyi;Liu Shupeng;Shang Yana;Wang Tingyun(Key Laboratory of Specialty Fiber Optics and Optical Access Networks,Joint International Research Laboratory of Specialty Fiber Optics and Advanced Communication,School of Communication and Information Engineering,Shanghai University,Shanghai 200444,China)

机构地区：[1]上海大学通信与信息工程学院,特种光纤与光接入网重点实验室,特种光纤与先进通信国际合作联合实验室,上海200444

出　　处：《中国激光》2022年第3期99-107,共9页Chinese Journal of Lasers

基　　金：国家自然科学基金(62027818);高等学校学科创新引智计划(111)资助项目(D20031)。

摘　　要：高精度的单细胞温度分布检测对于研究生命活动具有重要意义。为了实现高空间分辨率、高温度灵敏度的细胞测温,本研究团队基于原子力显微镜的扫描成像原理,将对温度敏感的量子点与音叉驱动的近场光纤探针相结合,提出了一种无损伤测量活细胞表面温度分布的方法;并以人脑星形胶质母细胞瘤细胞为研究对象,利用该方法检测了吞金纳米颗粒的固定细胞和未内吞金纳米颗粒的活细胞的温度分布。结果表明:在无金纳米颗粒作为附加热源的情况下,由活细胞内部产热引起的细胞表面最大温差超过0.5℃,而细胞与其外部环境间的温差大于2.5℃。所提活细胞无损测温方法的空间分辨率小于0.2μm,温度分辨率为0.16 nm/℃,为活细胞温度分布成像提供了新思路。Objective Temperature reflects vital life characteristics,and high precision measurement for single living cell temperature distribution is critical for studying living activities.Currently,the methods for measuring cell temperature are primarily divided into optical and nonoptical methods.In nonoptical methods,the temperature of the sample is characterized by an electrical signal.As a common nonoptical method,a thermocouple inserts the tip into a cell,causing cell damage.Furthermore,the tip’s large size results in low spatial resolution.Scanning thermal microscopy(SThM),which is based on the principle of atomic force microscopy(AFM),implements a smaller thermocouple probe to achieve nondestructive scanning with high spatial resolution.However,in a liquid environment,the electrical noise generated by the probe of the method prevents development in living cell temperature measurement.Optical temperature methods are mainly based on the traditional far-field optical detection technology,which obtains low spatial resolution.Furthermore,the fluorescence method,as the most common optical temperature measurement method,introduces luminescent molecules into the cell.However,the effect of molecules on the cell cannot be neglected.In this study,to achieve cell temperature measurement of high spatial resolution and high-temperature sensitivity,based on the scanning probe principle of AFM,we propose a nondestructive method for measuring the cellular surface temperature distribution in vivo.Methods The method proposed in this study for cell temperature measurement is based on near-field optics and the temperature characteristics of quantum dots fluorescence(Fig.1).Near-field optics,in contrast to far-field optics,resolves the diffraction limit and achieves optical resolution of less than half the illumination wavelength.Near-field optical detection is primarily based on the probe.The probe used in this study is an aperture near-field optical fiber probe.Under the feedback control of the tuning fork,the probe approaches the

关 键 词：生物光学 光纤传感器 近场光纤探针 量子点 测温 活细胞 

分 类 号：TN253[电子电信—物理电子学]