高效液相色谱-一测多评法测定肝爽颗粒中虎杖苷、白藜芦醇、氧化芍药苷、芍药苷和柴胡皂苷a、b_(1)、d  

Determination of polydatin,resveratrol,oxypaeoniflorin,paeoniflorin,saikosaponin a,saikosaponin b_(1),and saikosaponin d in Ganshuang Granules by HPLC-QAMS

作　　者：杨雯 张永州 杨本霞[3] YANG Wen;ZHANG Yong-zhou;YANG Ben-xia(Kaifeng Food and Drug Inspection Institute,Kaifeng 475000,China;Department of Pharmacy,Huaihe Hospital,Henan University,Kaifeng 475000,China;Chemistry Room,Henan Food and Drug Inspection Institute,Zhengzhou 450008,China)

机构地区：[1]开封市食品药品检验所,河南开封475000 [2]河南大学淮河医院药学部,河南开封475000 [3]河南省食品药品检验所化学室,河南郑州450008

出　　处：《现代药物与临床》2022年第2期285-290,共6页Drugs & Clinic

摘　　要：目的建立高效液相色谱-一测多评法(HPLC-QAMS)测定肝爽颗粒中虎杖苷、白藜芦醇、氧化芍药苷、芍药苷和柴胡皂苷a、b_(1)、d的方法。方法采用Agilent SB-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱;检测波长分别为306 nm(0~17 min,测定虎杖苷和白藜芦醇)、230 nm(17~28 min,测定氧化芍药苷和芍药苷)、210 nm(28~55 min,测定柴胡皂苷a、b_(1)、d);体积流量1.0 mL/min;柱温30℃;进样量10μL。以芍药苷为内参物,建立其他6个成分的相对校正因子(RCF)计算。结果虎杖苷、白藜芦醇、氧化芍药苷、芍药苷和柴胡皂苷a、b_(1)、d分别在6.37~159.25、4.91~122.75,1.88~47.00、7.99~199.75、0.56~14.00、2.19~54.75、2.87~71.75μg/mL线性关系良好,平均回收率分别为100.08%、98.95%、97.87%、99.52%、96.97%、98.88%、99.07%,RSD值分别为0.72%、1.1%、1.4%、0.99%、1.2%、1.2%、0.84%。肝爽颗粒中各成分的一测多评法所得结果与外标法结果无明显差异。结论所建立的HPLC-QAMS法可用于肝爽颗粒中虎杖苷、白藜芦醇、氧化芍药苷、芍药苷和柴胡皂苷a、b_(1)、d的同时测定。Objective To establish an high performance liquid chromatography-quantitative analysis of multi-component by single marker(HPLC-QAMS)method for determination of polydatin,resveratrol,oxypaeoniflorin,paeoniflorin,saikosaponin a,saikosaponin b_(1),and saikosaponin d in Ganshuang Granules.Methods Agilent SB-C_(18)(250 mm×4.6 mm,5μm)was used as the column with acetonitrile-0.1%phosphoric acid as the mobile phase by gradient elution.The detection wavelength were 306 nm for polydatin and resveratrol,230 nm for oxypaeoniflorin and paeoniflorin,and 210 nm for saikosaponin a,saikosaponin b_(1),and saikosaponin d.The flow rate was 1.0 m L/min,the column temperature was 30℃,and the injection volume was 10μL.With paeoniflorin as the internal reference,the relative correction factors(RCF)of the other six constituents were calculated,and the content were determined.Results Polydatin,resveratrol,oxypaeoniflorin,paeoniflorin,saikosaponin a,saikosaponin b_(1),and saikosaponin d showed good linear relationships within the ranges of 6.37—159.25,4.91—122.75,1.88—47.00,7.99—199.75,0.56—14.00,2.19—54.75,2.87—71.75μg/mL,whose average recoveries(RSDs)were 100.08%(0.72%),98.95%(1.1%),97.87%(1.4%),99.52%(0.99%),96.97%(1.2%),98.88%(1.2%),and 99.07%(0.84%),respectively.There was no significant difference on components contents between the quantitative results of HPLC-QAMS and those of external standard method(ESM).Conclusion HPLC-QAMS method can be used for the simultaneous determination of polydatin,resveratrol,oxypaeoniflorin,paeoniflorin,saikosaponin a,saikosaponin b_(1),and saikosaponin d in Ganshuang Granules.

关 键 词：肝爽颗粒 虎杖苷 白藜芦醇 氧化芍药苷 芍药苷 柴胡皂苷A 高效液相色谱-一测多评法 

分 类 号：R286.02[医药卫生—中药学]