2020年福州哨点医院5岁以下住院腹泻儿童A组轮状病毒全基因组分析  

Whole genome characterization of group A rotavirus among children under 5 years of age with acute diarrhea in Fuzhou sentinel hospital in 2020

作　　者：蒋弘萌 吴冰珊[2,3] 黄枝妙 翁育伟 林卫东[4] 郑奎城 Jiang Hongmeng;Wu Bingshan;Huang Zhimiao;Weng Yuwei;Lin Weidong;Zheng Kuicheng(The School of Public Health,Fujian Medical University,Fuzhou 350122,China;Fujian Provincial Key Laboratory of Zoonosis Research,Fujian Provincial Center for Disease Control and Prevention,Fuzhou 350001,China;Fujian Academy of Preventive Medicine,Fuzhou 350001,China;Fuzhou Children’s Hospital,Fuzhou 350004,China)

机构地区：[1]福建医科大学公共卫生学院,福州350122 [2]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州350001 [3]福建省预防医学研究院,福州350001 [4]福州市儿童医院,福州350004

出　　处：《中华实验和临床病毒学杂志》2022年第1期21-28,共8页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科技重大专项(2017ZX10103008)。

摘　　要：目的了解2020年福州市哨点医院5岁以下腹泻住院儿童粪便样本中A组轮状病毒(group A rotavirus,RVA)的基因组特征。方法通过ELISA对腹泻住院儿童粪便样本进行RVA筛查,采用RT-PCR方法扩增RVA阳性样本的11个基因节段并进行Illumina二代测序,使用MEGA等生物学软件对重要基因片段进行序列分析和抗原位点分析。结果成功获得20株RVA全基因组序列,包括4种G/P基因组合G9P[8](55%)、G3P[8](25%)、G8P[8](15%)以及G2P[4](5%),全基因组中DS-1-like重配株比例占40%(8/20)。相同型别的RVA序列同源性高,且与现今世界流行的毒株亲缘关系近。VP7、VP4和NSP4片段氨基酸位点变异情况复杂,重要抗原位点存在多处氨基酸变异情况发生。结论在福州地区首次检测到G3P[8]型和G8P[8]型DS-1-like重配株,VP7、VP4和NSP4片段抗原位点上存在变异。鉴于不常见DS-1-like重配株的检出和多个重要抗原位点变异现象的出现,有必要对RVA全基因组特征进行持续监测,为疫情防控和疫苗的免疫策略提供科学依据。Objective To understand the genome characteristics of group A rotavirus(RVA)strains among hospitalized children under 5 years of age with acute diarrhea in Fuzhou sentinel hospital in 2020.Methods The ELISA method was used for screening RVA-positive stool samples of hospitalized children under 5 years of age,then 11 gene segments of RVA-positive samples were sequenced and typed after amplification by RT-PCR,and their homology and phylogenetic analysis were performed by Molecular Evolutionary Genetics Analysis(MEGA).Results Twenty RVA whole genome sequences were successfully obtained,including 4 kinds of G/P gene combinations-G9P[8](55%),G3P[8](25%),G8P[8](15%)and G2P[4](5%).DS-1-like reassortant strains accounted for 40%of the whole genomes.Strains of the same type have high sequence homology,are closely related to the virus strains that currently circulating in the world.There are mutations at multiple important antigenic sites on VP7,VP4 and NSP4 fragments.The variation of amino acid substitutions of VP7,VP4 and NSP4 fragments is complicated,and there are many amino acid substitutions in the antigenic regions.Conclusions G3P[8]and G8P[8]DS-1-like reassortants were detected for the first time in Fuzhou,amino acid substitutions were observed in the antigenic regions of the VP7,VP4 and NSP4 gene.Due to the emergence of uncommon DS-1-like reassortant strains and multiple important antigenic regions substitutions,it is necessary to continuously monitoring genome characteristics of RVA strains to provide scientific evidence for the pandemic prevention and vaccine immunization strategies.

关 键 词：A组轮状病毒 全基因组 基因重配 

分 类 号：R725.1[医药卫生—儿科]