福建省2014—2019年麻疹病毒基因型监测情况分析  

Measles virus genotyping surveillance in Fujian province from 2014 to 2019

作　　者：李东[1] 张苏晗 陈致飞[1] 杨秀惠[1] 郑凝旋 张海荣 潘伟毅[1] 周勇[1] Li Dong;Zhang Suhan;Che Zhifei;Yang Xiuhui;Zheng Ningxuan;Zhang Hairong;Pan Weiyi;Zhou Yong(Fujian Provincial Key Laboratory of Zoonosis Research,Fujian Center for Disease Control and Prevention,Fuzhou 350001,China)

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病重点实验室,福州350001

出　　处：《中华实验和临床病毒学杂志》2022年第1期46-52,共7页Chinese Journal of Experimental and Clinical Virology

基　　金：福建省科技创新平台建设项目(2019Y2001)。

摘　　要：目的分析福建省2014—2019年不同基因型麻疹野病毒流行情况,为制定后续麻疹防控策略,完成消除麻疹目标提供参考。方法收集福建省2014—2019年麻疹发病监测数据及各地市上送的麻疹疑似病例咽拭子样本,采用逆转录-聚合酶链反应扩增麻疹病毒N蛋白羧基端450个核苷酸序列,对扩增产物进行序列鉴定和分析。结果2014—2019年福建省共报告疑似麻疹病例1102例,年发病率为0.47/10万;实验室共接收咽拭子标本884份,获得除疫苗株外麻疹病毒N蛋白羧基端450个核苷酸序列179条,其中110条为H1基因型麻疹毒株序列,43条为B3基因型麻疹毒株序列,26条为D8基因型麻疹毒株序列。福建省H1基因型麻疹病毒属于H1a基因亚型,存在两个分支,有19种不同的变异序列毒株,部分毒株与其他国家和地区的麻疹毒株高度同源(99.8%~100.0%);福建省B3基因型麻疹病毒存在6个变异序列毒株,与国内外流行的B3型麻疹毒株之间核苷酸同源性为98.4%~100.0%,其中部分毒株与2018年的香港HongKong.C HN/35.18/B3毒株同源性为100.0%,与美国、日本、菲律宾等国的B3基因型毒株高度同源(99.8%);而福建省D8基因型麻疹毒株存在5个变异序列毒株,部分毒株与2014年越南的HoChiMinh.VNM/11.14/MV95毒株、2019年日本的MVs/KobeC.JPN/28.19/19MR69毒株、2018年的泰国MVs/Samut_Sakhon.THA/8.18毒株高度同源。结论2014—2019年福建省共发现三种不同基因型别的麻疹野病毒(H1、B3、D8型)流行传播,其中H1a基因型依然是福建省的本土流行基因型,B3和D8基因型麻疹病毒为福建省输入性基因型毒株,并引起有限的传播流行。结果提示福建省近年来麻疹本土病例虽有所减少,但境外输入病例的风险在增大,后续应加强境外输入病例的监测。Objective To analyze the prevalence of different genotypes of wild measles virus in Fujian province from 2014 to 2019.Methods The information of suspected measles cases and the throat swab specimens of the cases in Fujian province from 2014 to 2019 were collected.Reverse transcription-polymerase chain reaction was applied to amplify the 450 nucleotides of the 3-terminal of the nucleoprotein gene.Phylogenetic tree was constructed to identify and analyze the target sequence.Results A total of 1102 suspected measles cases were reported in Fujian province from 2014 to 2019,with an annual incidence rate of 0.47/100000.Totally 884 throat swabs were tested in the laboratory,and 179 nucleotide sequences were obtained except vaccine strain.Sequence analysis showed that 110 measles genotypes were H1,43 were B3 and 26 were D8.Nucleotide homology analysis showed that the H1a genotype measles virus in Fujian province distributed in two branches,divided into nine different sequence variants.Some of the variants were highly homologous(99.8%-100.0%)with the measles strains in other regions and countries.There were six different sequence variants of B3 genotype measles virus in Fujian province.The nucleotide homology between the B3 genotype measles virus in Fujian province and the domestic and foreign prevalent B3 strains was 98.4%-100.0%.The D8 genotype measles strain in Fujian province had five different sequence variants,some of which were highly homologous with the strains from Vietnam,Japan and Thailand.Conclusions Three different genotypes of measles viruses(H1,B3 and D8)prevailed in Fujian province from 2014 to 2019,among which H1 genotype was still the local endemic genotype,while B3 and D8 genotypes were imported genotypes in Fujian province.The result indicated that although the local cases of measles were less in Fujian province in recent years,the risk of imported cases from abroad was increasing,and the surveillance of imported cases from abroad should be strengthened.

关 键 词：麻疹病毒 B3基因型 D8基因型 病原学监测 

分 类 号：R511.1[医药卫生—内科学]