北京地区呼吸道感染住院儿童中人博卡病毒流行病学和基因进化分析  

Epidemiology and genotypic diversity of human Bocavirus in pediatric patients with acute respiratory infection in Beijing

作　　者：张骞[1] 王超[1] 黄益曼 麻粉莲[1] 郑丽舒[1] Zhang Qian;Wang Chao;Huang Yiman;Ma Fenlian;Zheng Lishu(NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing100052,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,国家卫生健康委员会医学病毒与病毒病重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2022年第1期65-70,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：科技重大专项(2018ZX10102001,2018ZX10713002)。

摘　　要：目的分析北京地区呼吸道感染住院儿童人博卡病毒1型(human bocavirus 1,HBoV1)的流行病学特征,阐明遗传进化特点。方法采用real-time PCR方法对2017—2019年北京友谊医院呼吸道感染住院患儿的2848份鼻咽抽吸物样本进行HBoV1核酸检测,结合临床信息进行流行病学分析;利用巢氏PCR方法扩增HBoV1的NP1和VP1基因,分析序列同源性;构建VP1的最大分支可信度树和种群进化图,进行时间进化分析。结果2848份样本中检出HBoV1阳性样本90份,感染率为3.16%,5岁以下患儿居多(93.33%,84/90)。HBoV1感染全年可见,10月份病例数最多,检出率为7.23%(18/249)。HBoV1阳性病例多与其他呼吸道病毒混合感染(48.89%,44/90),临床症状多为咳嗽和发热。巢氏PCR扩增获得NP1区序列55条和VP1序列47条,核酸同源性分别为98.9%~100%和99.1%~100%。HBoV1 VP1时间进化分析显示本研究获得的HBoV1基因序列出现在两个进化分支上,HBoV1种群动态平稳。结论HBoV1是导致北京地区儿童呼吸道感染的常见病毒之一,其基因进化相对稳定,但仍需持续监测。Objective To investigate the epidemiological characteristics of human bocavirus 1(HBoV1)and to analyze the genetic variation.Methods A total of 2848 nasopharyngeal aspirate(NPAs)specimens were collected from hospitalized children with acute respiratory tract infections(ARTI)in Beijing Friendship Hospital from April 2017 to March 2019,and HBoV1 was detected by quantitative real-time PCR.Epidemiological analysis was carried out based on the clinical information of the patients.The nested PCR method was used to amplify the NP1 and VP1 genes of HBoV1 for homology analysis.Maximum clade credibility tree(MCC tree)and genetic polymorphism map were constructed to analyze the time evolution of HBoV1 VP1.Results HBoV1 was detected in 90(3.16%)of 2848 NPAs,most(93.33%,84/90)HBoV1-positive cases were among children<5 years of age.HBoV1 could be detected throughout the year with a higher prevalence 7.23%(18/249)in October.Of the 90 HBoV1-infected cases,the main clinical symptoms were fever and cough,44(48.89%)were co-infected with other respiratory viruses;55 NP1 sequences and 47 VP1 sequences were obtained by nested PCR amplification,phylogenetic analysis showed that the nucleotide homology was 98.9%~-100%and 99.1%~-100%,respectively.MCC tree showed that the HBoV1 VP1 gene sequence obtained in this study appeared in two adjacent clades,the gene evolution was stable.Conclusions HBoV1 is one of the common viruses that cause respiratory infection among children in Beijing.HBoV1 genetic evolution is relatively stable,but it still needs to be monitored continuously.

关 键 词：呼吸道感染 人博卡病毒 基因进化 

分 类 号：R725.6[医药卫生—儿科] R181.3[医药卫生—临床医学]