2011年甘肃省幼儿急疹病例中人疱疹病毒6型感染情况及病毒基因特征分析  

Viral infection and gene characteristics of human herpesvirus 6 in exanthema subitum cases in Gansu province,China in 2011

作　　者：周杨子 刘建锋[2] 赵晓虹[2] 张燕[1] 崔爱利[1] 陈瑛[2] Zhou Yangzi;Liu Jianfeng;Zhao Xiaohong;Zhang Yan;Cui Aili;Chen Ying(WHO WPRO Regional Reference Laboratory of Measles and Rubella,NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Centers for Disease Control and Prevention,Beijing 102206;Gansu Center for Disease Control and Prevention,Lanzhou 730020 China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,世界卫生组织西太平洋地区麻疹/风疹参比实验室,国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206 [2]甘肃省疾病预防控制中心,兰州730020

出　　处：《中华实验和临床病毒学杂志》2022年第1期71-76,共6页Chinese Journal of Experimental and Clinical Virology

基　　金："十三五"科技重大专项(2018ZX10713002,2018ZX10713001-003)。

摘　　要：目的阐明2011年甘肃省幼儿急疹病例中人疱疹病毒6型(human herpesvirus-6,HHV-6)感染情况及病毒基因特征。方法应用实时荧光定量PCR(Quantitative Real-time PCR,q-PCR)方法对2011年甘肃省收集的169份幼儿急疹病例的咽拭子标本进行HHV-6初筛,对HHV-6病毒核酸阳性标本进行U90基因PCR扩增、序列测定以及病毒基因特征分析。结果在169份幼儿急疹病例标本中,有60份标本检测为HHV-6病毒核酸阳性,获得了10条HHV-6B U90基因序列。HHV-6阳性病例主要集中在2岁以下婴幼儿,病例年龄中位数为9月龄。通过系统发育分析发现,HHV-6B可分为分支a和b。其中分支a又可进一步分为3个亚分支(亚分支a-1、a-2和a-3),不同亚分支之间呈现一定的地域聚集性。本研究获得的10条HHV-6B序列分散在系统发育树的不同分支上,分属于亚分支a-1和a-2以及分支b。在10条序列中未发现基因重组现象。结论甘肃省存在着多个HHV-6B分支/亚分支病毒同时流行,病毒呈现出序列多样性。通过本研究首次阐明了甘肃地区幼儿急疹病例中HHV-6B基因特征,填补了我国HHV-6B分子流行学研究的空白,同时也为全球HHV-6B研究积累了数据。Objective To clarify the infection status and gene characteristics of human herpesvirus 6(HHV-6)in exanthema subitum cases in Gansu province in 2011.Methods Throat swabs collected from 169 cases with exanthema subitum in Gansu province in 2011 were tested by the quantitative real-time PCR method.PCR amplification and sequence determination for U90 gene were performed for HHV-6 nucleic acid positive specimens and then the characteristics of U90 gene were analyzed in this study.Results Sixty out of 169 samples from exanthema subitum cases were positive for HHV-6 nucleic acid,and 10 sequences of HHV-6B U90 gene were obtained.HHV-6 positive cases were mainly concentrated in infants under 2 years of age,with a median age of 9 months.Phylogenetic analysis showed that the sequences of HHV-6B could be divided into clades a and b.And then the clade a could be further divided into three lineages(lineage a-1,a-2 and a-3)with geographical clustering.The 10 HHV-6B sequences obtained in this study were scattered in different branches of the phylogenetic tree,belonging to lineages a-1 and a-2,and clade b.No gene recombination was found in these 10 sequences.Conclusions Multiple HHV-6B clades/lineages were circulating in Gansu province,and the virus strains showed genetic polymorphism.Through this study,the gene characteristics of HHV-6B circulating in Gansu province were firstly clarified,which not only filled the gap of the HHV-6B molecular epidemiology in China,but also accumulated the data for global HHV-6B research.

关 键 词：幼儿急疹 人疱疹病毒6型B亚型 基因特征 

分 类 号：R725.1[医药卫生—儿科]