大鼠静脉-静脉体外膜肺氧合模型的建立与评价  

Establishment and evaluation of rat venovenous extracorporeal membrane oxygenation model

作　　者：黄健 张佑恒 张荣智[2] 李鉴 程行东 王石雄[1] 翟科蓉 吴向阳[1] 高秉仁[1] 李勇男[1] Huang Jian;Zhang Youheng;Zhang Rongzhi;Li Jian;Cheng Xingdong;Wang Shixiong;Zhai Kerong;Wu Xiangyang;Gao Bingren;Li Yongnan(Department of Cardiac Surgery,Lanzhou University Second Hospital,Lanzhou 730030,China;Department of Anesthesiology,Lanzhou University Second Hospital,Lanzhou 730030,China;Department of General Surgery,Lanzhou University Second Hospital,Lanzhou 730030,China)

机构地区：[1]兰州大学第二医院心脏外科,730030 [2]兰州大学第二医院麻醉科,730030 [3]兰州大学第二医院普通外科,730030

出　　处：《中华实验外科杂志》2022年第2期372-374,共3页Chinese Journal of Experimental Surgery

基　　金：甘肃省青年科技基金计划项目(20JR10RA760);兰州大学第二医院萃英学子科研培育计划项目(CYXZ2021-04);兰州大学第二医院院内引进人才计划(YJRCKYQDJ-2021-02)。

摘　　要：目的建立一种简易的大鼠静脉-静脉体外膜肺氧合(VV ECMO)模型。方法选用16只健康SD雄性大鼠[(350±50) g], 按随机数字表法分为假手术组(6只)和VV ECMO组(10只)。5%七氟烷诱导麻醉后, 20G静脉留置针进行大鼠气管插管, 连接小动物呼吸机, 全程用2%七氟烷维持麻醉。24G静脉留置针置入大鼠右侧股动脉, 连接生命体征监护仪进行监护。随后经大鼠右侧颈静脉顺势插入一种特制5.5 Fr的三腔管, 远心端的20G管腔作为VV ECMO环路的静脉引流端, 中间的22G管腔作为补液通道, 近心端的22G管腔作为VV ECMO环路的灌注端。大鼠VV ECMO辅助时间为3 h, 期间实时监测大鼠血压、心率, 并在术前、术中1 h、术中2 h及术后检测血气。组间比较采用t检验。结果 VV ECMO组中除1只因出血及静脉引流不畅, 在麻醉状态下直接死亡, 其余均在实验结束后在麻醉状态下进行处死。大鼠在转机60 min和120 min时动脉氧分压(PaO2)[(243.23±19.12)、(226.89±21.32) mmHg(1 mmHg=0.133 kPa)]均高于转机前[(104.34±12.13) mmHg], 差异有统计学意义(t=14.612、11.893, P<0.05)。动脉二氧化碳分压(PaCO_(2))[(34.43±4.21)、(35.22±2.31) mmHg], 血红蛋白(Hb)[(9.24±0.83)、(8.13±1.14) g/dL], 红细胞比容(Hct)[(30.62±0.81)%、(29.23±0.71)%]均低于转机前[(42.38±3.18) mmHg、(14.13±0.62) g/dL、(41.11±1.22)%], 差异有统计学意义(t=3.893、4.834、12.317、11.642、18.577、21.445, P<0.05)。结论本研究建立的简易大鼠VV ECMO模型为相关疾病的研究提供良好的模型基础。Objective To establish a simple model of rat venovenous extracorporeal membrane oxygenation(VV ECMO).Methods Totally,16 healthy male SD rats weighing(350±50)g were selected and divided into sham group(n=6)and VV ECMO group(n=10).After 5%sevoflurane induced anesthesia,the rats were intubated with 20G vein detained needle and connected to a small animal ventilator,and anesthesia was maintained with 2%sevoflurane throughout the whole process.The 24G vein detained needle was inserted into the right femoral artery of rats and connected with vital signs monitor for monitoring.A special 5.5 Fr three-cavity tube was inserted on the right jugular vein of rats,with the 20G lumen as the venous drainage end of the VV ECMO loop,the 22G lumen as the fluid supply channel and 22G lumen as the perfusion end of the ECMO loop.The VV ECMO assisted time of rats was 3 h,during which the blood pressure and heart rate of rats were monitored in real time,and blood gas was detected preoperatively,1 and 2 h intraoperatively and postoperatively.T test was used for measurement data.Results In the VV ECMO group,one animal died directly under anesthesia due to bleeding and poor venous drainage,and the rest were executed under anesthesia after the experiment.The arterial oxygen pressure(PaO_(2))[(243.23±19.12),(226.89±21.32)mmHg(1 mmHg=0.133 kPa)]at 60 min and 120 min with VV ECMO was higher than that before[(104.34±12.13)mmHg],and the difference was statistically significant(t=14.612,11.893,P<0.05).Arterial partial pressure of carbon dioxide(PaCO2)[(34.43±4.21),(35.22±2.31)mmHg],hemoglobin(Hb)[(9.24±0.83),(8.13±1.14)g/dL],and hematocrit(Hct)[(30.62±0.81)%,(29.23±0.71)%]were lower than those before[(42.38±3.18)mmHg,(14.13±0.62)g/dL,(41.11±1.22)%],and the differences were statistically significant(t=3.893,4.834,12.317,11.642,18.577,21.445,P<0.05).Conclusion The simple rat VV ECMO model established in this study provides a good model basis for the study of related diseases.

关 键 词：大鼠 体外膜肺氧合 

分 类 号：R563.9[医药卫生—呼吸系统] R-332[医药卫生—内科学]