Metagenomic insights into the profile of antibiotic resistomes in sediments of aquaculture wastewater treatment system  

作　　者：Jianfei Chen Yuyin Yang Xinshu Jiang Yanchu Ke Tao He Shuguang Xie 

机构地区：[1]State Key Joint Laboratory of Environmental Simulation and Pollution Control,College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China [2]State Key Joint Laboratory of Environment Simulation and Pollution Control(SKJLESPC),Beijing Key Laboratory for Emerging Organic Contaminants Control(BKLEOC),School of Environment,POPs Research Center,Tsinghua University,Beijing 100084,China [3]South China Institute of Environmental Sciences(SCIES),Ministry of Ecology and Environment(MEE),Guangzhou 510655,China

出　　处：《Journal of Environmental Sciences》2022年第3期345-355,共11页环境科学学报（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China(No.51778006);the High-performance Computing Platform of Peking University。

摘　　要：To meet the rapidly growing global demand for aquaculture products,large amounts of antibiotics were used in aquaculture,which might accelerate the evolution of antibiotic-resistant bacteria(ARB)and the propagation of antibiotic genes(ARGs).In our research,we revealed the ARGs profiles,their co-occurrence with mobile genetic elements(MGEs),and potential hosts in sediments of a crab pond wastewater purification system based on metagenomic analysis.The residual antibiotic seems to increase the propagation of ARGs in the crab pond,but there was no clear relationship between a given antibiotic type and the corresponding resistance genes.The effect of aquaculture on sediment was not as profound as that of other anthropogentic activities,but increased the relative abundance of sulfon-amide resistance gene.A higher abundance of MGEs,especially plasmid,increased the po-tential ARGs dissemination risk in crab and purification ponds.Multidrug and sulfonamide resistance genes had greater potential to transfer because they were more frequently carried by MGEs.The horizontal gene transfer was likely to occur among a variety of microorgan-isms,and various ARGs hosts including Pseudomonas,Acinetobacter,Escherichia,and Klebsiella were identified.Bacterial community influenced the composition of ARG hosts,and Pro-teobacteria was the predominant hosts.Overall,our study provides novel insights into the environmental risk of ARGs in sediments of aquaculture wastewater treatment system.

关 键 词：Metagenomic sequencing AQUACULTURE Antibiotic resistome Antibiotic resistance genes(ARGs) Mobile genetic elements(MGEs) ARG hosts 

分 类 号：X714[环境科学与工程—环境工程]