光敏水凝胶免疫识别严重急性呼吸综合征冠状病毒2 Spike蛋白的快速纳米检测方法  

Rapid Nano-Detection Method of Photosensitive Hydrogel Immunorecognition of Spike Protein of SARS-CoV-2

作　　者：段醒妹 陈小华[1,2,3] 帅明 DUAN Xingmei;CHEN Xiaohua;SHUAI Ming(School of Medicine,University of Electronic Science and Technology of China,Chengdu,Sichuan,China 610054;Sichuan Academy of Medical Sciences·Sichuan Provincial People′s Hospital,Chengdu,Sichuan,China 610072;Personalized Drug Therapy Key Laboratory of Sichuan Province,Chengdu,Sichuan,China 610072)

机构地区：[1]电子科技大学医学院,四川成都610054 [2]四川省医学科学院·四川省人民医院,四川成都610072 [3]个体化药物治疗四川省重点实验室,四川成都610072

出　　处：《中国药业》2022年第7期71-74,共4页China Pharmaceuticals

基　　金：四川省医学重点学科(实验室)及重点专科立项项目[20PJ097];四川省科技计划项目[20YYJC1516]。

摘　　要：目的建立严重急性呼吸综合征冠状病毒2(SARS-CoV-2)Spike(S)蛋白的快速检测方法。方法基于抗原-抗体免疫识别反应、纳米技术、光敏水凝胶技术,制备同时偶联抗体和光敏剂的纳米粒子溶液,SARS-CoV-2表面的S蛋白可与纳米粒子上的抗体形成牢固的非共价结合,去除其余干扰性结合后,在波长为405 nm的蓝光和光引发剂Irgacure 2959的作用下,S蛋白可使甲基丙烯酰化明胶(GelMA)水凝胶溶液由液态转变为固态。结果制备的检测溶液中,S蛋白抗体质量为50~100 mg,疏基-Irgacure 2959质量为5~10 mg。同时,当GelMA水凝胶溶液的浓度为5%~30%(m/V)时,检测效果较好。结论该方法生产成本低,检测效率高,易于贮存和运输,对设备和操作人员的依赖性低,可用于快速检测SARS-CoV-2表面的S蛋白。Objective To establish a rapid detection method for the Spike(S)protein of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods Based on antigen-antibody immunorecognition response,nanotechnology and photosensitive hydrogel technology,nanoparticle solution with simultaneous coupling of antibody and photosensitizer was prepared.The S protein on the surface of SARS-CoV-2 could form a strong non-covalent binding with the antibody on the nanoparticle,and after the remaining interfering binding was removed,gelatin methacryloyl(GelMA)hydrogel solution would be changed from liquid to solid under the action of blue light of 405 nm and photoinitiator Irgacure 2959 due to the S protein.Results In the prepared detection solution,the mass of S protein antibody was 50-100 mg and the mass of HS-irgacure 2959 was 5-10 mg.At the same time,when the concentration of GelMA hydrogel solution was 5%-30%(m/V),the detection effect was good.Conclusion The method has low production cost,high detection efficiency,easy storage and transportation,low dependence on equipment and operator,which can be used for rapid detection of the S protein on the surface of SARS-CoV-2.

关 键 词：严重急性呼吸综合征冠状病毒2 SPIKE蛋白 抗原-抗体免疫识别反应 光敏水凝胶技术 纳米技术 快速检测 

分 类 号：R446.5[医药卫生—诊断学]