载bpV(PIC)的神经干细胞联合神经营养素-3壳聚糖支架参与创伤性颅脑损伤后脑神经修复的研究  

The study of neural stem cells carrying bpV(PIC)combined with neurotrophin 3 chitosan scaffold in the repair of brain nerves after traumatic brain injury

作　　者：李军 管义祥 丁锦荣 刘小江 管诚 LI Jun;GUAN Yixiang;DING Jinrong;LIU Xiaojiang;GUAN Cheng(Department of Neurosurgery,Haian People′s Hospital,Haian 226600,China)

机构地区：[1]海安市人民医院神经外科,江苏海安226600

出　　处：《实用医学杂志》2022年第4期446-451,共6页The Journal of Practical Medicine

基　　金：江苏省卫生健康委科研项目(编号:Z2019033)。

摘　　要：目的探究载牛细小病毒bpV(PIC)的神经干细胞(neural stem cells,NSCs)联合神经营养素3(neurotrophin 3,NT-3)壳聚糖支架对创伤性颅脑损伤(traumatic brain injury,TBI)后脑神经修复的影响。方法将90只大鼠随机分为对照组、TBI组、TBI+NSCs组、TBI+bpV(PIC)-NSCs组、TBI+NT-3壳聚糖组和TBI+bpV(PIC)-NSCs+NT-3组(n=15)。通过撞击构建TBI模型,在撞击后植入NSCs、bpV(PIC)-NSCs、NT-3壳聚糖或者负载bpV(PIC)-NSCs的NT-3壳聚糖。通过mNSS评分和水迷宫实验评估神经功能。通过HE染色和BrdU染色评估脑组织损伤和神经元新生情况。结果TBI组的mNSS评分、脑组织损伤程度、新生神经元数量显著高于对照组,目标象限停留时间、穿越平台次数显著低于对照组(P<0.05)。TBI+NSCs组的mNSS评分和脑组织损伤程度显著低于TBI组,而目标象限停留时间、穿越平台次数和新生神经元数目显著高于TBI组(P<0.05)。TBI+bpV(PIC)-NSCs组的mNSS评分和脑组织损伤程度显著低于TBI+NSCs组,而目标象限停留时间、穿越平台次数和新生神经元数目显著高于TBI+NSCs组(P<0.05)。TBI+bpV(PIC)-NSCs+NT-3组的mNSS评分和脑组织损伤程度显著低于TBI+bpV(PIC)-NSCs组,而目标象限停留时间、穿越平台次数和新生神经元数目显著高于TBI+bpV(PIC)-NSCs组(P<0.05)。结论bpV(PIC)-NSCs联合NT-3壳聚糖支架可显著促进TBI大鼠的神经修复。Objective To explore the effects of neural stem cells(NSCs)carrying bovine parvovirus bpV(PIC)combined with neurotrophin 3(NT-3)chitosan scaffold on the nerves repair after traumatic brain injury(TBI).Methods 90 rats were randomly divided into control group,TBI group,TBI+NSCs group,TBI+bpV(PIC)-NSCs group,TBI+NT-3 chitosan group and TBI+bpV(PIC)-NSCs+NT-3 Group(n=15).The TBI model was constructed by impact,and NSCs,bpV(PIC)-NSCs,NT-3 chitosan or NT-3 chitosan loaded with bpV(PIC)-NSCs were implanted after impact.Neural function was evaluated by mNSS score and water maze test.HE staining and BrdU staining were used to evaluate brain tissue damage and neuron regeneration.Results The mNSS score,the degree of brain damage,and the number of new neurons in the TBI group were significantly higher than those of the control group,and the target quadrant residence time and the number of crossing platforms were significantly lower than those of the control group(P<0.05).The mNSS score and the degree of brain damage in the TBI+NSCs group were significantly lower than those in the TBI group,while the target quadrant residence time,the number of crossing platforms and the number of new neurons were significantly higher than those in the TBI group(P<0.05).The mNSS score and the degree of brain damage in the TBI+bpV(PIC)-NSCs group were significantly lower than those in the TBI+NSCs group,while the target quadrant residence time,the number of crossing platforms,and the number of newborn neurons were significantly higher than those in the TBI+NSCs group(P<0.05).The mNSS score and the degree of brain damage in the TBI+bpV(PIC)-NSCs+NT-3 group were significantly lower than those in the TBI+bpV(PIC)-NSCs group,while the target quadrant residence time,the number of crossing platforms,and the number of newborn neurons were significantly higher In the TBI+bpV(PIC)-NSCs group(P<0.05).Conclusion The combination of bpV(PIC)-NSCs and NT-3 chitosan scaffold can significantly promote nerve repair in TBI rats.

关 键 词：创伤性颅脑损伤 神经干细胞 牛细小病毒 壳聚糖支架 

分 类 号：R338[医药卫生—人体生理学]