奈达铂对肿瘤患者骨髓巨核细胞及血小板相关凋亡蛋白影响的研究  

The effect of Nedaplatin on bone marrow megakaryocytes and platelet-associated apoptotic protein in tumor patients

作　　者：韩忠丽 于柏清 李和义 尤奎雨 王武龙[1] Han Zhongli;Yu Baiqing;Li Heyi(The Second Affiliated Hospital of Baotou Medical College,Baotou,Inner Mongolia 014030)

机构地区：[1]包头医学院第二附属医院,内蒙古包头014030

出　　处：《基层医学论坛》2022年第7期4-6,共3页The Medical Forum

基　　金：包头医学院科学研究基金项目-青苗计划(BYJJ-QM201934)。

摘　　要：目的探讨奈达铂对肿瘤患者骨髓巨核细胞及血小板相关凋亡蛋白的影响。方法选取包头医学院第二附属医院2020年1月—2020年6月使用奈达铂化疗后第7~14天,并发生Ⅱ~Ⅲ度血小板抑制的恶性肿瘤患者30例作为研究对象,纳入研究组;另选取同期健康人群30例,纳入对照组。对实验标本取材和处理,培养巨核细胞;流式细胞仪检测PS膜外表达,了解可溶性P-选择素的释放、Caspase-3和Caspase-9的表达以及Bci-XL和Bak蛋白的相互作用。结果研究组PS膜外表达阳性率及可溶性P选择素均低于对照组,差异有统计学意义(P<0.05),在保存7 d的血小板中,研究组中BCL-XL和Bak蛋白的结合量高出对照组的2.6倍,且研究组中Caspase-3、caspase-9活性表达形式明显小于对照组,差异有统计学意义(P<0.05)。结论奈达铂对恶性肿瘤患者血小板下降的细胞凋亡机制与Caspase-3、caspase-9活性形式减少有关,同时受BCL-XL和Bak蛋白结合量升高的影响,对指导临床用药、保证临床治疗效果具有十分重要的参考价值。Objective To investigate the effect of nedaplatin on bone marrow megakaryocytes and platelet-related apoptotic proteins in tumor patients.Methods Thirty patients with malignant tumors withⅡtoⅢdegree platelet inhibition in our hospital from January 2020 to June 2020 on the 7-14 days after chemotherapy with nedaplatin were selected as the study objects,and they were included in the study group,and another selection During the same period,30 healthy people were included in the control group.The experimental specimens were collected and processed,and megakaryocytes were cultured.Flow cytometry was used to detect PS extra-membrane expression to understand the release of soluble P-selectin,Caspase-3 and Caspase-9.Expression and interaction of Bci-XL and Bak protein.Results The positive rate of PS extra-membrane expression and soluble P-selectin in the study group were lower than those in the control group.The difference was statistically significant(P<0.05).Among the platelets stored for 7 days,the levels of BCL-XL and Bak protein in the study group The binding amount was 2.6 times higher than that of the control group,and the percentages of the active forms of caspase-3 and caspase-9 in the study group were significantly less than those in the control group,and the difference was statistically significant(P<0.05).Conclusion The apoptosis mechanism of nedaplatin on platelet decline in patients with malignant tumors is related to the reduction of the active forms of Caspase-3 and Caspase-9,and is also affected by the increase of BCL-XL and Bak protein binding.It has very important reference value for guiding clinical medication and ensuring clinical treatment effects.

关 键 词：肿瘤 骨髓巨核细胞 奈达铂 血小板相关凋亡蛋白 膜外表达 

分 类 号：R453[医药卫生—治疗学]