新风胶囊对佐剂关节炎大鼠滑膜M1/M2巨噬细胞极化的影响  

Effect of Xinfeng Capsule on Polarization of Synovial M1/M2 Macrophages in Rats with Adjuvant Arthritis

作　　者：赵磊 万磊[2] 刘健[2] 黄传兵[2] 马熙檬 朱子衡 ZHAO Lei;WAN Lei;LIU Jian;HUANG Chuan-bing;MA Xi-meng;ZHU Zi-heng(Anhui University of Traditional Chinese Medicine,Hefei 230038,China;The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Anhui 230031,China)

机构地区：[1]安徽中医药大学,安徽合肥230038 [2]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《云南中医中药杂志》2022年第1期62-66,共5页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：国家自然科学基金面上项目(81973655);安徽省高校自然科学研究项目(KJ2020A0397);科技部国家重点研发计划中医药现代化研究重点专项(2018YFC1705204);全国中医药创新骨干人才培训项目(国中医药人教函[2019]128号);安徽中医药大学校基金项目(2018zrzd17);安徽中医药大学第一附属医院院内基金(2020yfyzc25、2020yfyzc55)项目资助。

摘　　要：目的观察新风胶囊对佐剂关节炎(AA)大鼠滑膜M1/M2巨噬细胞极化的影响。方法将大鼠随机均分为正常(NC)组、模型(MC)组和新风胶囊(XFC)组,每组10只,除正常组外,MC组和XFC组构建成AA大鼠模型。致炎后第12 d开始给药,给药30 d后,检测各组大鼠关节炎指数(AI),电镜观察滑膜超微结构,ELISA检测血清细胞因子的表达,流式细胞术检测大鼠外周血炎症极化标志物CD86+细胞、CD206+细胞的表达。结果与NC组相比,MC组大鼠AI、IL-1、CD86+细胞的表达升高,IL-10、CD206+细胞的表达降低差异均具有统计学意义(P<0.05,P<0.01);与MC组大鼠相比,XFC组大鼠AI、IL-1、CD86+细胞的表达降低,IL-10、CD206+细胞的表达升高差异均具有统计学意义(P<0.05,P<0.01)。结论新风胶囊可能通过调节M1/M2巨噬细胞极化改善佐剂关节炎大鼠关节炎症。Objective:To observe the effect of Xinfeng Capsule on the polarization of synovial M1/M2 macrophages in adjuvant arthritis(AA)rats.Methods:The rats were randomly divided into normal(NC)group,model(MC)group and Xinfeng Capsule(XFC)group,10 rats per group.Except for the normal group,the MC group and XFC group were made into AA rat models.The administration was started on the 12th day after the inflammation,and 30th day after the administration,and the arthritis index(AI)of each group was measured.The ultrastructure of the synovial membrane was observed under electron microscope,the expression of serum cytokines was measured by ELISA,and the expression of CD86+ cells and CD206+ cells in the peripheral blood of the rats was measured by flow cytometrym,which are markers of inflammatory polarization.Results:Compared with that of the NC group,the expression of AI,IL-1 and CD86+ cells of the MC group increased,and the expression of IL-10 and CD206+ cells decreased,and the difference was statistically significant(P<0.05,P<0.01)and compared with that of the MC group,the expression of AI,IL-1,and CD86+ cells of the XFC group decreased,and the differences of the increased expression of IL-10 and CD206+ cells were statistically significant(P<0.05,P<0.01).Conclusion:Xinfeng Capsule may improve the joint inflammation of adjuvant arthritis rats by regulating the polarization of M1/M2 macrophages.

关 键 词：佐剂关节炎 巨噬细胞极化 新风胶囊 

分 类 号：R285.5[医药卫生—中药学]