复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响  

Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma

作　　者：刘蕊 范宁建[2] 王英曼 宋洁 张岩 LIU Rui;FAN Ningjian;WANG Yingman;SONG Jie;ZHANG Yan(Dept.of Chemoradiotherapy,North China University of Science and Technology Affiliated Hospital,Hebei Tangshan 063000,China;Dept.of Spine,the Second Hospital of Tangshan,Hebei Tangshan 063000,China)

机构地区：[1]华北理工大学附属医院肿瘤放化疗科,河北唐山063000 [2]唐山市第二医院脊柱三科,河北唐山063000

出　　处：《中国医院用药评价与分析》2022年第3期300-303,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：河北省中医药管理局科研计划项目(No.2019170)。

摘　　要：目的:探讨复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响。方法:体外培养多发性骨髓瘤RPMI8226细胞系,采用四甲基偶氮唑盐法检测不同浓度复方苦参注射液对细胞增殖的影响,采用流式细胞仪检测细胞周期和凋亡,采用酶联免疫吸附法检测白细胞介素6(IL-6)的表达水平。结果:0.5、1、2、5和10 mg/mL的复方苦参注射液均有抑制骨髓瘤RPMI8226细胞增殖的作用,且呈时间和剂量依赖性。5、10 mg/mL的复方苦参注射液可显著促进细胞凋亡,并阻滞细胞周期于G_(1)期。复方苦参注射液治疗后,细胞IL-6表达水平明显下调。结论:复方苦参注射液能抑制骨髓瘤细胞的增殖,诱导凋亡,其机制可能与抑制IL-6的表达有关。OBJECTIVE:To probe into the effects of compound Kushen injection on proliferation and apoptosis of RPMI8226 cells in multiple myeloma.METHODS:RPMI8226 cells in multiple myeloma was subcultured in vitro.The effects of different concentrations of compound Kushen injection on cell proliferation were detected by Methyl thiazolyl tetrazolium,and cell cycle and apoptosis were detected by flow cytometry.The expression of interleukin-6(IL-6)was detected by enzyme-linked immunosorbent assay.RESULTS:Compound Kushen injection of 0.5 mg/mL,1 mg/mL,2 mg/mL,5 mg/mL and 10 mg/mL could inhibit the proliferation of RPMI8226 cells in multiple myeloma in a time and dose dependent manner.Compound Kushen injection of 5 mg/mL and 10 mg/mL could significantly promote cell apoptosis and block cell cycle in G_(1) phase.The expression of IL-6 decreased significantly after the treatment of compound Kushen injection.CONCLUSIONS:Compound Kushen injection can inhibit the proliferation and induce the apoptosis of RPMI8226 cells,and its mechanism may be related to the inhibition of IL-6 expression.

关 键 词：多发性骨髓瘤 复方苦参注射 增殖 白细胞介素6 

分 类 号：R932[医药卫生—生药学] R979.1[医药卫生—药学]