CHI3L1基因多态性与支气管哮喘患儿吸入性糖皮质激素疗效的关系  

Correlation Between CHI3L1 Gene Polymorphism and Efficacy of Inhaled Glucocorticoid in Children with Bronchial Asthma

作　　者：陈瑞东 谢菲[1] 赵洁 徐春艳 昝春香 田文秋 伊文霞[1] 岳彬[1] CHEN Ruidong;XIE Fei;ZHAO Jie;XU Chunyan;ZAN Chunxiang;TIAN Wenqiu;YI Wenxia;YUE Bin(Dept.of Pediatrics,Cangzhou Central Hospital,Hebei Cangzhou 061000,China;Dept.of Children’s Health Prevention,Cangzhou Central Hospital,Hebei Cangzhou 061000,China)

机构地区：[1]沧州市中心医院儿科,河北沧州061000 [2]沧州市中心医院儿保科,河北沧州061000

出　　处：《中国医院用药评价与分析》2022年第3期327-331,共5页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：河北省医学科学研究项目(No.20211650)。

摘　　要：目的:探讨几丁质酶3类似物1(CHI3L1)基因多态性与支气管哮喘患儿吸入性糖皮质激素疗效的关系。方法:选取2019—2020年该院收治的142例支气管哮喘患儿为观察组,接受吸入性糖皮质激素治疗;另选取同期92例体检健康儿童为对照组。采用Mass ARRAY-IPLEX质谱阵列法检测CHI3L1基因rs883125、rs4950928和rs10399805位点单核苷酸多态性,麦邦MAS-99肺功能检测仪检查观察组患儿治疗前后用力肺活量(FVC)、第1 s用力呼气容积(FEV_(1))和FEV_(1)/FVC。比较观察组和对照组患儿CHI3L1基因rs883125、rs4950928和rs10399805位点基因型频率,观察组不同疗效患儿CHI3L1基因rs883125、rs4950928和rs10399805位点基因型频率,观察组CHI3L1基因rs883125、rs4950928和rs10399805位点各基因型患儿治疗前后肺功能指标差值。结果:观察组患儿rs883125位点GG基因分布频率明显高于对照组,rs4950928位点CC基因分布频率明显低于对照组,CG基因分布频率明显高于对照组,差异均有统计学意义(P<0.05);两组患儿rs10399805位点基因型频率比较,差异无统计学意义(P>0.05)。142例支气管哮喘患儿吸入性糖皮质激素治疗12周后,良好控制率为69.01%(98/142);疗效良好组患儿rs883125位点GG基因分布频率明显低于疗效不良组,rs4950928位点CC基因分布频率明显高于疗效不良组,差异均有统计学意义(P<0.05);不同疗效组患儿rs10399805位点基因型频率比较,差异无统计学意义(P>0.05)。观察组rs883125位点CC型、CG型患儿治疗前后FVC、FEV_(1)和FEV_(1)/FVC的差值明显高于GG型;rs4950928位点CC型患儿治疗前后FVC、FEV_(1)和FEV_(1)/FVC的差值明显高于CG型、GG型,GG型患儿治疗前后FVC、FEV_(1)和FEV_(1)/FVC的差值明显高于CG型,上述差异均有统计学意义(P<0.05)。观察组CHI3L1基因rs10399805位点各基因型患儿治疗前后FVC、FEV_(1)、FEV_(1)/FVC的差值比较,差异无统计学意义(P>0.05)。结论:CHI3L1基因rs883125、rs4950928位�OBJECTIVE:To probe into the correlation between chitinase 3-like 1(CHI3L1)gene polymorphism and efficacy of inhaled glucocorticoid in children with bronchial asthma.METHODS:Totally 142 children with bronchial asthma treated in this hospital from 2019 to 2020 were extracted as the observation group and received inhaled glucocorticoid therapy.And 92 healthy children under physical examination during the same period were selected as the control group.Single nucleotide polymorphisms at rs883125,rs4950928,and rs10399805 of CHI3L1 gene were detected by Mass ARRAY-IPLEX mass spectrometry array method.Forced vital capacity(FVC),forced expiratory volume in one second(FEV_(1)),FEV_(1)/FVC before and after treatment were detected in the observation group by micron MAS-99 pulmonary function tester.The genotype frequency of rs883125,rs4950928 and rs10399805 of CHI3L1 gene was compared between the observation group and the control group.The genotype frequency of rs883125,rs4950928 and rs10399805 of CHI3L1 gene was compared in the observation group with different curative effects.The difference of pulmonary function indicators at rs883125,rs4950928 and rs10399805 of CHI3L1 gene was compared in the observation group before and after treatment.RESULTS:The frequency of GG gene distribution at rs883125 was significantly higher in the observation group than in the control group,the frequency of CC gene distribution at rs4950928 was significantly lower than that in the control group,and the frequency of CG gene distribution was significantly higher than that in the control group,with statistically significant differences(P<0.05),and there was no difference in genotype frequency between two groups at rs10399805(P>0.05).After 12 weeks of inhaled glucocorticoid treatment in 142 children with bronchial asthma,the good control rate was 69.01%(98/142),the frequency of GG gene distribution at rs883125 in the good curative effect group was significantly lower than that in the poor curative effect group,and the CC gene distribution frequency

关 键 词：儿童 支气管哮喘 吸入性糖皮质激素 几丁质酶3类似物1 单核苷酸多态性 疗效 

分 类 号：R977.1[医药卫生—药品]