小麦CesA基因家族鉴定及特征分析  

Identification and Characteristic Analysis of Wheat CesA Gene Family

作　　者：吴兴阳 胡义锋 马东方[1] 刘易科 方正武[1] WU Xingyang;HU Yifeng;MA Dongfang;LIU Yike;FANG Zhengwu(College of Agriculture,Yangtze University,Jingzhou 434000,China;Food Crops Research Institute,Hubei Academy of Agricultural Sciences,Wuhan 430000,China)

机构地区：[1]长江大学农学院,湖北荆州434000 [2]湖北省农科院粮食作物研究所,湖北武汉430000

出　　处：《云南农业大学学报（自然科学版）》2022年第2期203-213,共11页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家重点研发计划项目(2018YFD0200506);国家自然科学基金项目(31501620)。

摘　　要：【目的】系统鉴定和分析小麦纤维素合成酶(CesA)基因家族成员,为进一步阐明小麦CesA基因家族的生物学功能奠定理论基础。【方法】利用生物信息学方法在小麦全基因组上系统鉴定小麦CesA (TaCesA)基因家族的成员。对小麦CesA基因的系统发育、染色体位置和转录组进行分析。预测TaCesAs基因结构、保守基序、顺式元件、蛋白质特征和亚细胞定位,并对小麦及其亚基因组供体中CesAs同源基因进行比对。【结果】共鉴定出21个TaCesA基因,分为3组(a、b和c)。基因结构分析发现TaCesA含有多个内含子,但部分基因非翻译区(UTR)结构存在缺失。TaCesA基因与其亚基因组供体关系保守。TaCesAs的上游包含45个与生物胁迫/非生物胁迫、生长发育和植物激素相关的元件,预示这些基因可能参与响应小麦的生物学功能。表达谱结果显示:TaCesA基因在缺磷、高温、低温、干旱、条锈病、白粉病和赤霉病等逆境胁迫中均有响应。【结论】小麦CesA基因具有保守的基因结构和蛋白结构,且在小麦抵御非生物和生物胁迫中起着重要作用。[Purpose]To systematically identified and analyzed wheat cellulose synthase(CesA)gene family members, laying a theoretical foundation for further elucidating their biological functions.[Methods]The wheat CesA(TaCesA) gene family members were comprehensively identified on the whole wheat genome using bioinformatics methods. The phylogeny, chromosome position, and transcriptome of TaCesA genes were analyzed. Then, TaCesAs gene structure, conserved motifs, ciselements, protein characteristics, and subcellular location were predicted. Moreover, CesA homologous genes between wheat and its subgenomic donors were compared. [Results]A total of 21 TaCesA genes were identified, divided into three groups(a, b and c). The TaCesAs contained multiple introns, but the untranslated region(UTR) structure of some genes was missing. The relationship between the TaCesA genes and their subgenome donors was conserved. The upstream of TaCesAs cover 45 cis-acting elements related to biological/abiotic stress, growth and development, and plant hormones, suggesting that these genes might be involved in the biological function respondings of wheat. The expression profile results showed the TaCesA genes were responsive to phosphorus deficiency, high temperature, low temperature, drought, stripe rust, powdery mildew, Fusarium graminearum, and other stresses. [Conclusion]The wheat CesA genes have conserved gene structure and protein structure, and they play an essential role in wheat resistance to abiotic and biological stresses.

关 键 词：小麦 纤维素合成酶(CesA) 生物信息学分析 基因表达谱 

分 类 号：S512.103.2[农业科学—作物学]