津巴布韦烟草丛顶病病原物的分子鉴定  

Molecular Identification of the Causative Agents of Tobacco Bushy Top Disease in Zimbabwe

作　　者：赵兴能[1,2,3] 张未 张丽芳[1,2,5] 徐萍[1,2] 李艳琼 张谊寒 陈海如 莫笑晗[2] ZHAO Xingneng;ZHANG Wei;ZHANG Lifang;XU Ping;LI Yanqiong;ZHANG Yihan;CHEN Hairu;MO Xiaohan(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Yunnan Academy of Tobacco Agricultural Science,Kunming 650021,China;Wenshan Branch of Yunnan Tobacco Company,Wenshan 663099,China;College of Life Sciences,Yunnan University,Kunming 650500,China;College of Bioresources and Food Engineering,Qujing Normal University,Qujing 655011,China;College of Agronomy and Life Sciences,Kunming University,Kunming 650214,China)

机构地区：[1]云南农业大学植物保护学院,云南昆明650201 [2]云南省烟草农业科学研究院,云南昆明650021 [3]云南省烟草公司文山州公司,云南文山663099 [4]云南大学生命科学学院,云南昆明650500 [5]曲靖师范学院生物资源与食品工程学院,云南曲靖655011 [6]昆明学院农学与生命科学学院,云南昆明650214

出　　处：《云南农业大学学报（自然科学版）》2022年第2期237-242,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：宁波大学农产品质量安全危害因子与风险防控国家重点实验室项目(KF20190107);中国烟草总公司云南省公司科技计划(2021530000242031,2021YKYZY03)。

摘　　要：【目的】鉴定津巴布韦烟草丛顶病病原复合体组成。【方法】采集津巴布韦烟草丛顶病样品,对其病原复合体进行分子鉴定。【结果】烟草丛顶病毒津巴布韦分离物(tobacco bushy top virus-Zimbabwe,TBTV-Zimbabwe)在基因组RNA核苷酸序列水平上与幽影病毒属(Umbravirus)其他成员的一致性为47.8%~94.6%,其中与埃塞俄比亚烟草丛顶病毒(Ethiopian tobacco bushy top virus,ETBTV)序列的一致性最高,为94.6%;与中国烟草丛顶病毒(tobacco bushy top virus,TBTV)序列的一致性仅为56.9%。分子系统进化分析表明:该病毒分离物与ETBTV同源性最高,为ETBTV的分离物,与TBTV为不同的病毒种。TBTV-Zimbabwe卫星RNA与ETBTV卫星RNA在基因组全长核苷酸序列水平上的一致性为93.9%,与GenBank数据库中除ETBTV卫星RNA之外的其他序列无同源性。研究还获得了津巴布韦烟草丛顶病病原复合体中马铃薯卷叶病毒(potato leafroll virus,PLRV)外壳蛋白基因部分核苷酸序列。【结论】津巴布韦烟草丛顶病病原复合体包含3种病毒组分,它们分别与埃塞俄比亚烟草丛顶病病原复合体的3种组分在病毒种的分类水平上一致。津巴布韦与中国烟草丛顶病病原物差异较大。[Purpose]To identify the components of the causative agents of tobacco bushy top disease in Zimbabwe. [Method]A tobacco bushy top diseased plant was collected from Zimbabwe,and molecular identification of the virus complex was conducted. [Results]The genomic nucleotide identities of TBTV-Zimbabwe with other umbraviruses were 47.8%-94.6%. TBTV-Zimbabwe had the highest identity of 94.6% with Ethiopian tobacco bushy top virus(ETBTV). TBTV-Zimbabwe had 56.9% identity with a Chinese isolate of tobacco bushy top virus(TBTV). Molecular phylogenetic analyses showed that TBTV-Zimbabwe was an isolate of ETBTV and it was distantly related to TBTV, which was a distinct virus species. The genomic nucleotide sequence identity between TBTV-Zimbabwe satellite RNA and ETBTV satellite RNA was 93.9%, and TBTV-Zimbabwe satellite RNA showed no homology with other sequences in the GenBank except ETBTV satellite RNA. Nucleotide sequence encoding partial coat protein of potato leafroll virus(PLRV) was determined in tobacco bushy top complex in Zimbabwe.[Conclusion]Three viral components were identified in tobacco bushy top disease complex in Zimbabwe. Each of them was identical to the components of tobacco bushy top disease complex in Ethiopia at the virus species level. The causative agents of Zimbabwean and Chinese tobacco bushy top disease were not the same.

关 键 词：津巴布韦 烟草丛顶病 病原复合体 卫星RNA 马铃薯卷叶病毒 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]