肺康益气颗粒对脂多糖诱导大鼠急性肺损伤保护作用及免疫作用研究  

Protective Effect of Feikang Yiqi Granule(肺康益气颗粒)on Lipopolysaccharide-induced Acute Lung Injury in Rats and Immunomodulatory Effect

作　　者：甘雨[1] 石岩[2] 乔敏[1] 朱竟赫 秦文艳[1] 张宏[1] 武晓琳[1] 范英兰[1] 于雪峰[1] GAN Yu;SHI Yan;QIAO Min;ZHU Jinghe;QIN Wenyan;ZHANG Hong;WU Xiaolin;FAN Yinglan;YU Xuefeng(The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110034,Liaoning,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China)

机构地区：[1]辽宁中医药大学附属第二医院,辽宁沈阳110034 [2]辽宁中医药大学,辽宁沈阳110847

出　　处：《辽宁中医药大学学报》2022年第1期22-25,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家中医药管理局区域中医(肺病专科)诊疗中心项目;辽宁省自然科学基金计划项目(20180550124);辽宁省新型冠状病毒感染的肺炎疫情防控应急科研攻关项目(辽科发[2020]3号)。

摘　　要：目的探讨肺康益气颗粒对脂多糖(LPS)诱导的大鼠急性肺损伤(ALI)的保护作用及免疫调节作用,为新型冠状病毒肺炎(COVID-19)恢复期的临床治疗提供理论依据。方法采用气管内滴注LPS(5 mg/kg)构建ALI模型,将大鼠随机分为正常组,假手术组,模型组,肺康益气颗粒低(2.7 g/kg)、中(5.4 g/kg)、高(10.8 g/kg)剂量组,六君子丸组(1.62 g/kg)和地塞米松片组(1.08 mg/kg)。给药7 d后处死动物,摘取胸腺、脾、肺组织称重,计算脏器指数及肺组织湿/干重比,HE染色观察肺组织病理改变,ELISA法测定肺组织白细胞介素-1β(IL-1β)、前列腺素E2(PGE2)含量。采用腹腔注射环磷酰胺(50 mg/kg)法构建免疫功能低下模型,观察小鼠网状内皮系统吞噬能力。结果与模型组比较,肺康益气颗粒低剂量组脾指数、胸腺指数显著降低(P<0.05);肺康益气颗粒低、高剂量组左肺湿重、肺组织湿/干重比均显著降低(P<0.05或P<0.01);肺康益气颗粒中、高剂量组病理评分均显著降低(P<0.05或P<0.01);肺康益气颗粒低、中、高剂量组肺组织中IL-1β、PGE2含量显著降低(P<0.05或P<0.01);肺康益气颗粒中、高剂量组小鼠吞噬指数显著降低(P<0.05或P<0.01)。结论肺康益气颗粒能明显减轻LPS诱导的ALI大鼠肺水肿,缓解肺组织损伤及炎症反应,对急性肺损伤有一定的保护作用,且能提高机体的免疫功能,适用于COVID-19肺炎恢复期肺脾气虚证的治疗。Objective To explore the protective effect of Feikang Yiqi Granule(肺康益气颗粒)on lipopolysaccharide(LPS)-induced acute lung injury(ALI)in rats and its immunomodulatory effect,and to provide theoretical basis for the treatment of convalescent patients with corona virus disease 2019(COVID-19).Methods The ALI model was established by intratracheal instillation of LPS(5 mg/kg),and the rats were randomly divided into normal group,sham operation group,model group,low dose(2.7 g/kg),medium dose(5.4 g/kg),and high dose(10.8 g/kg)groups of Feikang Yiqi Granule,Liujunzi Pills(六君子丸,1.62 g/kg)group and dexamethasone tablets(1.08 mg/kg)group.After 7 days of administration,the thymus,spleen and lung tissues were weighed,and the organ index and lung tissue wet/dry weight ratio were calculated.The pathological changes of lung tissue were observed by HE staining,and the content of IL-1βand PGE2 in lung tissue was determined by ELISA.The immunosuppression model was established by intraperitoneal injection of cyclophosphamide(50 mg/kg),in order to observe the phagocytic ability of the mouse reticuloendothelial system.Results Compared with the model group,the spleen index and thymus index of the low-dose Feikang Yiqi Granule group were significantly reduced(P<0.05);the wet weight of the left lung and the wet/dry ratio of lung tissue were significantly decreased in the low and high-dose groups(P<0.05 or P<0.01);the pathological scores of the medium and high dose groups were significantly decreased(P<0.05 or P<0.01);the levels of IL-1βand PGE2 in the lung tissues of the low,medium and high dose groups were significantly reduced(P<0.05 or P<0.01);the phagocytic index of mice in the middle and high dose groups of Feikang Yiqi Granule was significantly reduced(P<0.05 or P<0.01).Conclusion Feikang Yiqi Granule could significantly reduce the pulmonary edema induced by LPS in ALI rats,relieve the lung tissue injury and inflammatory reaction,had a certain protective effect on acute lung injury,and could improve the immune f

关 键 词：急性肺损伤 新型冠状病毒肺炎 肺康益气颗粒 恢复期 免疫调节 

分 类 号：R563[医药卫生—呼吸系统]