树生黄单胞杆菌脂蛋白生物信息学分析  

Bioinformatics analysis of proteins with lipoprotein signal peptide in Xanthomonas arboricola

作　　者：喻红稠 覃悦 韩长志[1,3] YU Hongchou;QIN Yue;HAN Changzhi(College of Biodiversity Conservation,Southwest Forestry University;Graduate School of Southwest Forestry University;Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Kunming,Yunnan 650224,China)

机构地区：[1]西南林业大学生物多样性保护学院 [2]西南林业大学研究生院 [3]云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《福建农林大学学报（自然科学版）》2022年第2期230-237,共8页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(31960314);云南省“兴滇英才支持计划”青年人才专项(YNWR-QNBJ-2020-188)。

摘　　要：具有脂蛋白信号肽的蛋白参与细胞膜结构构建、细胞膜稳定性维护、信号转导和转运等过程,在维持细菌生理功能、致病性和耐药性等方面发挥重要作用.树生黄单胞杆菌是杧果生产中的重要病原之一,目前尚缺乏有关该菌脂蛋白信号肽蛋白的研究.本研究基于树生黄单胞杆菌的全基因组序列,通过LipoP v1.0在线程序分析,获取了175条具有脂蛋白信号肽的蛋白序列,并对这些蛋白开展了包括保守结构域、信号肽、跨膜区结构域、亚细胞定位和理化性质等的生物信息学分析.结果表明:144个脂蛋白氨基酸序列长度集中于100~500个之间;163条蛋白序列具有信号肽,仅有13条蛋白序列具有明显的保守结构域;90个蛋白定位在线粒体;这些蛋白在亲(疏)水性最强氨基酸残基及其位置方面存在较大的差异.此外,通过遗传关系分析进一步明确了与树生黄单胞杆菌中脂蛋白信号肽蛋白具有较高同源性和较近亲缘关系的其他物种,而大部分脂蛋白信号肽间的同源性并不高.Lipoprotein plays an important role in maintaining physiological functions,pathogenicity and drug resistance of bacteria via preserving cell membrane structure and transducing signal,etc.To elucidate the function of lipoprotein in Xanthomonas arboricola,one of the important pathogens in mango fruit production causing leaf spot disease,175 proteins with lipoprotein signal peptide were screened from the entire genome of X.arboricola through LipoP v1.0 online program.Then informations about conserved domains,signal peptides,transmembrane domains,subcellular localizations and physicochemical properties were predicted by bioinformatics approach.The results showed that the lengths of 144 lipoproteins ranged from 100 to 500.Among the 175 proteins with lipoprotein signal peptides,only 13 proteins consisted of obvious conserved domains,and 90 proteins were located in mitochondria.These proteins differed greatly in hydrophilic/hydrophobic amino acid residues and positions.Genetic relationship analysis was further conducted to identify species with lipoprotein homologous to their counterparts in X.arboricola,however,the homology between most proteins with lipoprotein signal peptides was not high.

关 键 词：脂蛋白信号肽 树生黄单胞杆菌 遗传关系 生物信息学 

分 类 号：S435.1[农业科学—农业昆虫与害虫防治]