Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan  

作　　者：Zhanpeng Shang Lulu Xu Yi Kuang Yan Lin Shuang Liu Long Sun Tao Bo Min Ye Xue Qiao 

机构地区：[1]State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China [2]School of Pharmaceutical Sciences,Guizhou Medical University,Guizhou,550025,China [3]Thermo Fisher Scientific,Beijing,100102,China

出　　处：《Journal of Pharmaceutical Analysis》2021年第6期717-725,共9页药物分析学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (Grant No.: 2018YFC1707304, 2018YFC1707301);Beijing Natural Science Foundation (Grant No.: JQ18027);National Natural Science Foundation of China (Grant No.: 81725023)。

摘　　要：Xiaoer-Feire-Kechuan(XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary inflammation.The complicated composition rendered its chemical analysis and effective-component elucidation.In this study,we combined quantitative analysis and bioactivity test to reveal the antiinflammatory constituents of XFK.First,UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were established and validated to quantify 35 analytes(covering 9 out of 11 herbs) in different XFK formulations.Parallel reaction monitoring mode built in Q-Orbitrap-MS was used to improve the sensitivity and selectivity.Then,anti-inflammatory activities of the 35 analytes were analyzed using in vitro COX-2 inhibition assay.Finally,major analytes forsythosides H,I,A(8-10),and baicalin(15)(total contents varied from 21.79 to 91.20 mg/dose in different formulations) with significant activities(inhibitory rate ≥ 80%) were proposed as the anti-inflammatory constituents of XFK.The present study provided an effective strategy to discover effective constituents of multi-herb formulas.

关 键 词：Multi-herb formulas Effective constituents Xiaoer-Feire-Kechuan formula(XFK) Parallel reaction monitoring(PRM) Cyclooxygenase-2(COX-2) 

分 类 号：R286.0[医药卫生—中药学]