水稻叶褐条斑病病原菌的分离鉴定与接种技术研究  

Isolation,Identification and Inoculation Techniques of Pathogenic Bacteria from Brown Leaf Streak in Rice

作　　者：赵茜[1,2] 姜树坤[2] 王立志[2] 杨贤莉 李明贤[2] 李忠杰[2] 迟立勇[2] 李锐[2] 吴建忠[3] 张丽艳[4] 李柱刚[2] ZHAO Qian;JIANG Shu-kun;WANG Li-zhi(Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086;Institute of Cultivation and Culture,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086)

机构地区：[1]黑龙江省农业科学院,黑龙江哈尔滨150086 [2]黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨150086 [3]黑龙江省农业科学院草业研究所,黑龙江哈尔滨150086 [4]黑龙江省农业科学院生物研究所,黑龙江哈尔滨150086

出　　处：《安徽农业科学》2022年第6期120-122,共3页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省自然科学基金(YQ2020C040);黑龙江省博士后基金(LBH-Z19207);黑龙江省农业科技创新工程项目(2018YYYF004,HNK2019CX14);黑龙江省省属科研院所科研业务费(CZKYF2021B005);黑龙江省农业科学院长期性基础性项目(2019CQJC002,2020CQJC002,2021CQJC002)。

摘　　要：在黑龙江稻区采集病样、分离病原菌的基础上,获得形态差异明显的20株水稻叶褐条斑病菌,利用ITS序列鉴定其均为Nigrospora oryzae,由此推断Nigrospora oryzae为当地主要的水稻黑孢菌种群。此外,依据水稻叶褐条斑病的生物学特征,摸索了人工接种鉴定技术。该研究结果为抗水稻叶褐条斑病的遗传育种研究奠定基础。In this study,20 strains of BLS disease were isolated and identified from rice as Nigrospora oryzae by internal transcribed spacer(ITS)sequence analysis.It was concluded that Nigrospora oryzae was the main population of rice melanospora in this area.In addition,we have explored the artificial inoculation and identification technique according to the biological characteristics of LBS in rice.The results of this study laid a foundation for the program of genetic breeding of resistance to BLS in rice.

关 键 词：稻黑孢菌 Nigrospora oryzae 分离与鉴定 标准化接种技术 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]