亚硝酸盐处理对PVY和TuMV的钝化作用研究  

Passivation of PVY and TuMV by Nitrite Treatment

作　　者：赵凯 王海娟 张荠丹 郭志慧 赵明敏[1] ZHAO Kai;WANG Hai-juan;ZHANG Ji-dan(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特010019 [2]乌兰察布市兴和县鄂尔栋镇综合保障和技术推广中心,内蒙古乌兰察布012000

出　　处：《安徽农业科学》2022年第6期129-131,148,共4页Journal of Anhui Agricultural Sciences

基　　金：内蒙古农业大学高层次人才引进科研启动项目(NDGCC-2016-23)。

摘　　要：为了探究化学试剂处理对植物病毒的钝化作用,以马铃薯Y病毒(potato virus Y,PVY)和芜菁花叶病毒(turnip mosaic virus,TuMV)为试验对象,在模式植物本氏烟上研究了亚硝酸盐处理对病毒的钝化作用。结果表明,亚硝酸盐处理PVY后,PVY在本氏烟植株上比清水处理的对照表现出发病症状减轻,甚至不发病现象。而清水处理的对照烟草对PVY病毒比较敏感,植株逐渐形成系统性侵染并最终坏死。亚硝酸盐处理TuMV后,未观察到病毒侵染的症状,也未检测到TuMV病毒。Western blot技术检测亚硝酸盐处理组病毒含量远低于清水处理组,接近健康水平。表明亚硝酸盐对PVY和TuMV具有一定的钝化作用。In order to explore the inactivation effect of chemical reagents on plant viruses,we used potato virus Y(PVY)and turnip mosaic virus(TuMV)as test objects.The inactivation effect of nitrite treatment on the virus was studied.The results of the study showed that after nitrite treatment of PVY,PVY on Ben’s tobacco plants showed a reduction in the onset of symptoms compared with the control treated with water,or even no onset.On the other hand,the control tobacco treated with clean water was more sensitive to PVY virus,and the plant gradually formed a systemic infection and eventually necrosis.After nitrite treatment of TuMV,no symptoms of virus infection were observed,and there was no detected TuMV virus.Western blot technology detected that the virus content of the nitrite treatment group was far lower than that of the water treatment group,which was close to a healthy level.The above results all showed that nitrite had a certain passivation effect on PVY and TuMV.

关 键 词：马铃薯Y病毒 芜菁花叶病毒 亚硝酸盐 钝化作用 

分 类 号：S432.41[农业科学—植物病理学]