山丹种子表面灭菌方法比较试验  

Comparative study on surface sterilization methods for Lilium pumilum seed

作　　者：邓煜 王蒙 李发虎[2] 李龙梅[2] 侯佳[2] DENG Yu;WANG Meng;LI Fahu;LI Longmei;HOU Jia(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China;College of Vocational and Technical,Inner Mongolia Agricultrual University,Salaqi 014109,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特010011 [2]内蒙古农业大学职业技术学院,内蒙古萨拉齐014109

出　　处：《北方农业学报》2022年第1期87-92,共6页Journal of Northern Agriculture

基　　金：内蒙古自治区教育厅高校科研项目(NJZY18066)。

摘　　要：【目的】筛选适宜的山丹种子表面灭菌方法,优化山丹无菌播种工艺。【方法】采用无菌播种方法对山丹种子进行培养,分别以1.0%NaClO和0.1%升汞作为消毒剂对山丹种子进行消毒,采用梯度时间(0~30 min)处理,培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,定期观察山丹种子发芽及生长情况。【结果】相同处理时间下,0.1%升汞处理污染率均低于1.0%NaClO处理。0.1%升汞处理下,山丹种子的发芽和生长情况优于1.0%NaClO处理。随着处理时间的延长,山丹种子萌发和生长情况呈现先增加后减少的趋势,以0.1%升汞处理15 min表现最佳,萌发率达97.78%,且幼苗生长质量优于其他处理。【结论】0.1%升汞处理15 min,山丹种子可以取得较高的萌发率和较好的幼苗生长质量。【Objective】Select the optimal seed surface sterilization method to improve Lilium pumilum′s aseptic seeding procedure.【Methods】Lilium pumilum seeds were cultured using aseptic sowing method.Seeds were disinfected with 1.0%NaClO or 0.1%HgCl;with gradient treatment duration(0-30 min).Seeds were then cultured on MS medium mixed with6-BA 1.0 mg/L and NAA 0.1 mg/L.The germination and growth were monitored regularly.【Results】The contamination rate of 0.1%HgCl;treatment was lower than that of 1.0%NaClO treatment at the same treatment time.Lilium pumilum seeds germination and growth were better with 0.1%HgCl;treatment than with 1.0%NaClO treatment.Seed germination and growth increased initially then declined as treatment duration was extended.The best treatment was 15 min of 0.1%HgCl;,which had a 97.78%germination rate and superior seedling growth than the other treatments.【Conclusion】Lilium pumilum seeds had higher germination rate and better seedling growth after being treated with 0.1%HgCl;for 15 min.

关 键 词：山丹种子 组织培养 表面灭菌 污染率 萌发率 诱导率 

分 类 号：S682.29[农业科学—观赏园艺]