HPLC法同时测定芩石利咽口服液中8种成分及其镇咳、抗炎活性研究  

Simultaneous determination of eight constituents in Qinshi Liyan Oral Liquid by HPLC and its antitussive and anti-inflammatory activities

作　　者：石美玲 王月洁 胡朝阳 李丽静[1] 鲍慧玮[1] SHI Mei-ling;WANG Yue-jie;HU Zhao-yang;LI Li-jing;BAO Hui-wei(College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117

出　　处：《中成药》2022年第3期712-717,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81274036);吉林省科技发展计划项目(20190304100yy);长春中医药大学校内基金项目(2019012)。

摘　　要：目的建立HPLC法同时测定芩石利咽口服液(黄芩、生石膏、水牛角等)中原儿茶酸、原儿茶醛、咖啡酸、芍药苷、连翘酯苷A、黄芩苷、木犀草素、汉黄芩素的含量,并研究其镇咳、抗炎活性。方法该药物的HPLC分析采用phenomenex柱(250 mm×4.6 mm,5μm);流动相甲醇-0.1%磷酸,梯度洗脱;体积流量1 mL/min;柱温30℃;检测波长238、254、322 nm。采用氨水致小鼠咳嗽模型、二甲苯致小鼠耳廓肿胀模型、蛋清致小鼠足肿胀模型,评价该药物药理活性。结果8种成分在各自范围内线性关系良好(r>0.9995),平均加样回收率97.81%~98.79%,RSD 0.84%~1.96%。与空白组比较,芩石利咽口服液中、高剂量组(7.8、15.6 g/kg)小鼠咳嗽次数、耳肿胀度、足肿胀度降低(P<0.05),咳嗽潜伏期延长(P<0.01)。结论该方法快速简便,重复性好,可用于镇咳、抗炎活性良好的芩石利咽口服液的质量控制。AIM To establish an HPLC method for the simultaneous content determination of protocatechuic acid, protocatechuic aldehyde, caffeic acid, paeoniflorin, phillyrin A, baicalin, luteolin and wogonin in Qinshi Liyan Oral Liquid(Scutellariae Radix, Gypsum Fibrosum, Bubali Cornu, etc.) and to study its antitussive and anti-inflammatory activities.METHODS The analysis of this drug was performed on a 30 ℃ thermostatic phenomenexcolumn(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of methanol-0.1%phosphoric acid flowing at 1 mL/minin a gradient elution manner, and the detection wavelengths were set at 238, 254, 322 nm. Ammonia-induced mouse cough model, xylene-induced mouse ear swelling model and albumen-induced mouse foot swelling model were adopted in the evaluation of this drug’s pharmacological activities.RESULTS Eight constituents showed good linear relationships within their own ranges(r>0.999 5), whose average recoveries were 97.81%-98.79%with the RSDs of 0.84%-1.96%. Compared with the blank group, Qinshi Liyan Oral Liquid medium-dose and high-dose groups(7.8, 15.6 g/kg) demonstrated decreased mouse cough frequency, ear swelling degree and foot swelling degree(P<0.05), and prolonged cough latency(P<0.01).CONCLUSION This rapid, simple and reproducible method can be used for the quality control of Qinshi Liyan Oral Liquid with good antitussive and anti-inflammatory activities.

关 键 词：芩石利咽口服液 化学成分 镇咳活性 抗炎活性 HPLC 

分 类 号：R927.2[医药卫生—药学]