诱导猪肺泡巨噬细胞CD163受体表达的细胞因子筛选  

Screening of Cytokines to Induce CD163 Receptor Expressions in Porcine Alveolar Macrophages

作　　者：刘源[1] 马卓媛 樊港 楚电峰 张仕强[1] 王妍[1] LIU Yuan;MA Zhuo-yuan;FAN Gang;CHU Dian-fen;ZHANG Shi-qiang;WANG Yan(College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China;State Key Laboratory of Genetically Engineered Veterinary Vaccines/Qingdao Yibang Biological Engineering Co.,Ltd,Qingdao,Shandong 266000,China)

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]动物基因工程疫苗国家重点实验室/青岛易邦生物工程有限公司,山东青岛266000

出　　处：《动物医学进展》2022年第4期12-18,共7页Progress In Veterinary Medicine

基　　金：动物基因工程疫苗国家重点实验室开放课题(AGVSKL-ZD-202009);国家自然科学基金项目(32072856)。

摘　　要：猪肺泡巨噬细胞(porcine alveolar macrophages,PAM)中的CD163受体作为明星分子不仅参与正常的生理活动,而且作为重要的病毒受体介导猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)入侵宿主靶细胞,因此其表达调控的研究备受关注。为了探讨巨噬细胞集落刺激因子(M-CSF)、粒细胞巨噬细胞集落刺激因子(GM-CSF)、转化生长因子β1(TGF-β1)、白介素10(IL-10)、白介素3(IL-3)和地塞米松(dexamethasone)及其组合对PAM中CD163受体表达的促进作用,通过RT-qPCR和免疫荧光染色在原代PAM中从mRNA和蛋白水平对不同因子及其组合中CD163受体的表达情况进行检测。结果显示,M-CSF、GM-CSF、TGF-β1、IL-10、IL-3和dexamethasone都能在一定范围内促进CD163受体的表达,通过组合发现dexamethasone+IL-10对于CD163受体表达的促进作用最为显著,为进一步开展CD163的表达调控研究奠定了基础。CD163receptor in PAM not only participates in normal physiological activities,but also acts as an important viral receptor to mediate porcine reproductive and respiratory syndrome virus(PRRSV)invading host target cells,so the study on the regulation of CD163expression is of great interest.To investigate the promotion of CD163in PAM by M-CSF,GM-CSF,TGF-β1,IL-10,IL-3and dexamethasone and their combinations,RT-qPCR and immunofluorescence staining were performed.The results showed that M-CSF,GM-CSF,TGF-β1,IL-10,IL-3and dexamethasone all promoted the expressions of CD163in a certain range.Among them,the combination of dexamethasone and IL-10promoted the expression of CD163significantly,which laid the foundation for the further investigation of the regulation of CD163.

关 键 词：肺泡巨噬细胞 CD163 地塞米松 白介素10 猪 

分 类 号：S852.4[农业科学—基础兽医学] S858.28[农业科学—兽医学]