板蓝根多糖在3D4/21/CD163细胞上对PRRSV感染过程的影响  

Effect of Isatidis Radix Polysaccharides on PRRSV Infection Process in 3D4/21/CD163 Cells

作　　者：刘建 苏科 朱世豪 樊俊洋 李倩楠 郝潇雅 杨明凡[1,2,3] 张红英 LIU Jian;SU Ke;ZHU Shi-hao;FAN Jun-yang;LI Qian-nan;HAO Xiao-ya;YANG Ming-fan;ZHANG Hong-ying(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou,Henan,450002,China;Key Laboratory for Animal-Derived Food Safety of Henan province,Zhengzhou 450000,China;Key Laboratory for Study and Evaluation of Chinese Veterinary Medicine of Zhengzhou City,Zhengzhou,Henan,450000,China)

机构地区：[1]河南农业大学动物医学院,河南郑州450000 [2]河南省动物性食品安全重点实验室,河南郑州450000 [3]郑州市中兽药研究与评价重点实验室,河南郑州450000

出　　处：《动物医学进展》2022年第4期52-57,共6页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31972731)。

摘　　要：以永生化的传代猪肺泡巨噬细胞(3D4/21/CD163)作为靶细胞,研究板蓝根多糖(IRPS)对猪繁殖与呼吸综合征病毒(PRRSV)的体外抗病毒效果。研究IRPS的最佳给药方式及其抑制病毒增殖的环节,分别以预防作用、直接杀灭作用和治疗作用来测定IRPS抑制PRRSV增殖的最佳作用方式和药物浓度;从IRPS对PRRSV的吸附、入侵环节的影响确定IRPS抑制病毒感染的具体环节,用荧光定量PCR和间接免疫荧光对IRPS的抗PRRSV作用进行验证。结果显示,当药物浓度为0.062 5 mg/mL时对PRRSV的直接杀灭作用和预防作用效果最好,显著降低PRRSV在细胞中的拷贝数;IRPS主要是在病毒入侵细胞的环节发挥作用。In this study, immortalized porcine alveolar macrophages(3 D4/21/CD163) were used as target cells to study the antiviral effect of Isatidis Radix polysaccharides(IRPS) on PRRSV in vitro.In order to study the best administration mode of IRPS and the link of inhibiting virus proliferation, the best action mode and drug concentration of IRPS inhibiting PRRSV proliferation were determined by preventive effect, direct killing effect and therapeutic effect, respectively;From the influence of IRPS on the adsorption and invasion of PRRSV,the specific links of IRPS inhibiting virus replication we are determined.The anti-PRRSV effect of IRPS was verified by quantitative real-time PCR and indirect immunofluo rescence assay(IFA).The results showed that when the concentration of the drug was 0.0625 mg/mL,the direct killing effect and preventive effect on PRRSV were the best, and the copy number of PRRSV in cells was significantly reduced.IRPS mainly plays a role in the process of virus invading cells.

关 键 词：猪繁殖与呼吸综合征 板蓝根多糖 抗病毒作用 

分 类 号：S852.651[农业科学—基础兽医学] S853.92[农业科学—兽医学]