新疆铁门关市部分地区散养奶牛嗜血支原体和泰勒虫感染情况的调查  

Prevalence of Hemoplasmas and Theileria in Scattered-feeding Dairy Cattle in Some Regions of Tiemenguan,Xinjiang

作　　者：郎平 陈宏伟[1] 姜云[2] 罗慧丽 赵爱云[1] 张秀萍[1] 齐萌[1] LANG Ping;CHEN Hong-wei;JIANG Yun;LUO Hui-li;ZHAO Ai-yun;ZHANG Xiu-ping;QI Meng(College of Animal Science,Tarim University/Key Laboratory of Tarim Animal Science and Technology of Xinjiang Production and Construction Group,Alar,Xinjiang,843300,China;The Second Division of Animal Husbandry and Veterinary Station of Xinjiang Production and Construction Corps,Tiemenguan,Xinjiang,841023,China)

机构地区：[1]塔里木大学动物科学学院/兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [2]新疆生产建设兵团第二师畜牧兽医工作站,新疆铁门关841023

出　　处：《动物医学进展》2022年第3期124-128,共5页Progress In Veterinary Medicine

基　　金：新疆生产建设兵团中青年科技创新领军人才计划项目(2018CB034);新疆生产建设兵团第二师铁门关市科技攻关项目(2018NYGG03);新疆生产建设兵团塔里木畜牧科技重点实验室开放课题项目(HS201904)。

摘　　要：为了解新疆铁门关市3个镇散养奶牛嗜血支原体和泰勒虫感染分布,选取85头1岁~2岁奶牛进行血液样本采集,提取其全基因组DNA,用PCR检测血液DNA样本,对阳性样本进行基因碱基序列分析。结果表明,奶牛嗜血支原体和泰勒虫的总感染率为23.5%(20/85),天湖镇和河畔镇奶牛总感染率分别为39.5%(15/38)和26.3%(5/19),双丰镇奶牛未检测出血液病原。鉴定出3种病原,温氏支原体(n=13)、牛嗜血支原体未定种(n=11)和环形泰勒虫(n=7),病原混合感染率为9.4%(8/85);温氏支原体、牛嗜血支原体未定种和环形泰勒虫均存在3种基因型序列。说明新疆铁门关市奶牛嗜血支原体和泰勒虫感染存在多样分布特征。To survey the prevalence and the species distribution of hemoplasmas and Theileria in the scattered-feeding dairy cattle in Tiemenguan,Xinjiang,85 blood samples from 1-2 years old cows were collected for the extraction of genomic DNA,and detected pathogens by PCR and sequencing analysis of positive samples.The total infection rate of hemoplasmas and Theileria was 23.5%(20/85),with 39.5%(15/38)in Tianhu town,26.3%(5/19)in Hepan town,negative in Shuangfeng town.Mycoplasma wenyonii(n=13),Candidatus Mycoplasma haemobos(n=11)and Theileria annulata(n=7)were identified,and the mixed infection rate of them was 9.4%(8/85).Three genotypes were found in each of the three pathogens from the Neighbor-Joining phylogenetic tree.The results suggest that the infection of hemoplasmas and Theileria in dairy cows was common in Tiemenguan,with a diversification of genetic distribution characteristics.

关 键 词：嗜血支原体 泰勒虫 感染 聚合酶链反应 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学]