Development of an analytical method based on solid-phase extraction and LC-MS/MS for the monitoring of current-use pesticides and their metabolites in human urine  

作　　者：Beibei Gao Giulia Poma Govindan Malarvannan Catalina Dumitrascu Michiel Bastiaensen Minghua Wang Adrian Covaci 

机构地区：[1]Toxicological Centre,University of Antwerp,Wilrijk 2610,Belgium [2]Department of Pesticide Science,College of Plant Protection,State and Local Joint Engineering Research Center of Green Pesticide Invention and Application,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Environmental Sciences》2022年第1期153-163,共11页环境科学学报（英文版）

基　　金：supported by the China Scholarship Council oversea Ph D study program(No.201906850003);by the Exposome center of Excellence of the University of Antwerp(BOF grant,Antigoon ID no.41222)。

摘　　要：Pyrethroids,organophosphorus pesticides and fipronil have been listed as priority chemicals in human biomonitoring studies because of their wide use and potential health effects in humans.The determination of 13 pesticides,including pyrethroids(deltamethrin,cypermethrin,permethrin,cyfluthrin,bifenthrin),organophosphorus(chlorpyrifos,chlorpyrifosmethyl,and malathion),fipronil,neonicotinoids(imidacloprid,acetamiprid and thiacloprid)and triazole(prothioconazole),together with 13 corresponding metabolites in human urine samples was achieved by solid-phase extraction and analysis by liquid chromatography coupled to tandem mass spectrometry.All targeted compounds,except malathion dicarboxylic acid,were measured with a mean within-accuracy(n=5)of 71%–114%(RSD:1%–14%)and between-run(n=15)accuracy of 80%–118%(RSD:2%–14%).Limits of quantitation of the targeted analytes ranged from 0.1 to 16 pg/m L.The detection result of urine samples from 25 volunteers indicated that the detection frequencies of 3,5,6-trichloro-2-pyridinol(median:448 pg/m L),6-chloropyridine-3-carboxylic acid(median:193 pg/m L),2-methyl-3-phenylbenzoic acid(median:181 pg/m L),3-phenoxybenzoic acid(median:99 pg/m L),2-isopropyl-6-methyl-4-pyrimidinol(median:77 pg/m L),cyfluthrin(median:59 pg/m L),cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid(cis-DCCA,median:53 pg/m L),trans-DCCA(median:25 pg/m L),prothioconazole(median:21 pg/m L),imidacloprid(median:7 pg/m L),and prothioconazole-desthio(median:1 pg/m L)were>50%.The obtained results show that the validated method is suitable for the human biomonitoring of these currentuse pesticides and their metabolites.

关 键 词：PYRETHROIDS ORGANOPHOSPHORUS FIPRONIL NEONICOTINOIDS URINE BIOMONITORING 

分 类 号：X839.2[环境科学与工程—环境工程]