急性冠脉综合征患者血清胆固醇酯转运蛋白水平与CETP TaqIB基因多态性的关系研究  

A Study on the Relationship Between Serum Cholesteryl Ester Transfer Protein Level and CETP TaqIB Gene Polymorphism inPatients with Acute Coronary Syndrome

作　　者：曹程 王庆凯[1] 杨宝刚[1] 马明静[1] CAO Cheng;WANG Qingkai;YANG Baogang;MA Mingjing(Department of Cardiology,Hebei Cangzhou Integrated Traditional Chinese and Western Medicine Hospital,Cangzhou 061001,China)

机构地区：[1]河北省沧州中西医结合医院心内一科,河北沧州061001

出　　处：《标记免疫分析与临床》2022年第2期321-326,共6页Labeled Immunoassays and Clinical Medicine

基　　金：沧州市重点研发计划项目(编号:172302031)。

摘　　要：目的研究急性冠脉综合征患者血清胆固醇酯转运蛋白(CETP)水平与CETP TaqIB基因多态性的关系。方法在2020年1月至10月之间纳入75例急性冠脉综合征(ACS)作为ACS组,包含急性心肌梗死(MI)40例和不稳定型心绞痛(UAP)35例。另外纳入性别年龄相匹配的健康志愿者60例作为对照组。酶联免疫吸附试剂盒测定血清CETP,通过限制性片段长度多态性聚合酶链式反应分析CETP TaqIB基因多态性。比较2组血清CETP、脂质水平和CETP TaqIB基因多态性。结果2组基因型频率均符合Hardy-Weinberg平衡(P>0.05)。ACS组CETP TaqIB基因B1B1型分布频率高于对照组(P=0.023),且B1等位基因频率高于对照组(OR:0.555,95%CI:0.341~0.903,P=0.017)。与B1B1组比较,ACS患者B1B2、B2B2型HDL-C水平显著降低,血清CETP水平显著上升,均P<0.05。与B1B2型比较,B2B2型HDL-C水平显著降低,血清CETP水平显著上升,均P<0.05。经Pearson相关性分析,ACS患者血清CETP与TC呈正相关性(r_(s)=0.235),与HDL-C呈负相关性(r_(s)=-0.672,均P<0.05。结论鉴定TaqIB基因SNP能够较为准确地反映内环境脂质稳态,可能有助于对高危人群进行筛查。Objective To study the relationship between serum cholesteryl ester transfer protein(CETP)level and CETP TaqIB gene polymorphism in patients with acute coronary syndrome.Methods Between January and October,2020,75 cases of acute coronary syndrome(ACS)were included as the ACS group,including 40 cases of acute myocardial infarction(MI)and 35 cases of unstable angina(UAP).In addition,60 healthy volunteers with matching gender and age were included as the control group.The enzyme-linked immunosorbent assay kit was used to determine serum CETP,and the CETP TaqIB gene polymorphism was analyzed by restriction fragment length polymorphism polymerase chain reaction.We then compared serum CETP,lipid levels and CETP TaqIB gene polymorphism between the two groups.Results The frequencies of genotypes in both groups were in accordance with Hardy-Weinberg equilibrium(P>0.05).The frequency of B1B1 type distribution of CETP TaqIB gene in the ACS group was higher than that of the control group(P=0.023),and the frequency of B1 allele was higher than that of the control group(OR:0.555,95%CI:0.341-0.903,P=0.017).Compared with the B1B1 group,the levels of B1B2 and B2B2 HDL-C in ACS patients were significantly reduced,and serum CETP level was significantly increased(both P<0.05).Compared with type B1B2,the level of HDL-C of type B2B2 decreased significantly,and the level of serum CETP increased significantly(both P<0.05).According to Pearson correlation analysis,serum CETP of ACS patients was positively correlated with TC(r_(s)=0.235),while negatively correlated with HDL-C(r_(s)=-0.672,both P<0.05).Conclusion The identification of TaqIB gene SNP can more accurately reflect the internal environment lipid homeostasis,which is helpful for screening high-risk populations.

关 键 词：急性冠脉综合征 胆固醇酯转运蛋白 CETP TaqIB 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病]