宏基因组二代测序技术协助诊断3例无焦痂儿童恙虫病  

Diagnosis of 3 pediatric cases of scrub typhus without the eschar by metagenomics next-generation sequencing

作　　者：林威 林蓓蓓 唐震海 梁亚峰 潘国权 Lin Wei;Lin Beibei;Tang Zhenhai;Liang Yafeng;Pan Guoquan(Department of Pediatric Emergency and Critical Care Medicine,the Second Affiliated Hospital and Yuying Children′s Hospital of Wenzhou Medical University,Wenzhou 325000,China)

机构地区：[1]温州医科大学附属第二医院、育英儿童医院儿童重症与急诊医学科,温州325000

出　　处：《中华实用儿科临床杂志》2022年第3期210-213,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：浙江省自然科学基金(LY17H150004)。

摘　　要：目的对3例无焦痂恙虫病患儿的病原宏基因组二代测序技术(mNGS)检测结果进行分析,探讨其在临床的应用价值。方法回顾性分析。2018年6月至2019年10月温州医科大学附属第二医院、育英儿童医院收治的3例重症无焦痂儿童恙虫病的临床资料,2例为5岁男童,1例为6岁女童。3例患儿的外周血均进行mNGS检测。结果3例患儿经mNGS检测后均提示恙虫病东方体感染,且未并其他病原感染。例1死亡,例2和例3治愈出院。结论无焦痂恙虫病患儿早期诊断困难。对于临床感染性疾病,特别是早期无法确诊的疑难危重性感染性疾病,mNGS能够提供快速准确的病原学诊断支持,为精准治疗提供帮助。Objective To analyze the pathogen of 3 pediatric cases of scrub typhus without the eschar using metagenomics next-generation sequencing(mNGS),and the clinical application value of mNGS.Methods Clinical data of 3 pediatric cases of severe scrub typhus without the eschar in the Second Affiliated Hospital and Yuying Children′s Hospital of Wenzhou Medical University from June 2018 to October 2019 were analyzed retrospectively.Among them,2 cases were 5-year-old males and 1 case was 6-year-old female.Peripheral blood samples of 3 children were detected via mNGS technology.Results Through mNGS detection,all 3 children were diagnosed with Orientia tsutsugamushi infection and were not complicated with other pathogenic infections.Case 1 died,and case 2 and case 3 were cured.Conclusions Early diagnosis of scrub typhus without the eschar is difficult.Clinical infectious diseases,especially complicated and critical infectious diseases are difficult to be diagnosed at an early stage,mNGS can provide fast and accurate pathogenic diagnosis support for precise treatment.

关 键 词：恙虫病 焦痂 病原 宏基因组二代测序技术 儿童 

分 类 号：R725.3[医药卫生—儿科]