深圳儿童酿脓链球菌分离株多基因序列分型  

Multilocus sequence typing of Streptococcus pyogenes isolated from children in Shenzhen

作　　者：禹定乐[1] 梁云梅[2] 卢清华 赵瑞珍[1] 陈运生[3] 郑跃杰[1] 杨永弘[1,4] Yu Dingle;Liang Yunmei;Lu Qinghua;Zhao Ruizhen;Chen Yunsheng;Zheng Yuejie;Yang Yonghong(Department of Respiration,Shenzhen Children′s Hospital,Shenzhen 518038,China;Department of Pediatrics,Beijing Chaoyang Hospital Affiliated to Capital Medical University,Beijing 100043,China;Department of Laboratory,Shenzhen Children′s Hospital,Shenzhen 518038,China;Microbiology Laboratory,Beijing Children′s Hospital Affiliated to Capital Medical University,Beijing 100045,China)

机构地区：[1]深圳市儿童医院呼吸科,深圳518038 [2]首都医科大学附属北京朝阳医院儿科,北京100043 [3]深圳市儿童医院检验科,深圳518038 [4]首都医科大学附属北京儿童医院微生物实验室,北京100045

出　　处：《中华实用儿科临床杂志》2022年第4期295-298,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：广东省医学科研基金(A2021437);广东省高水平临床重点专科(深圳市配套建设经费)(SZGSP012);深圳市三名工程(SZSM201512030);深圳市医学重点学科建设经费(SZXK032);深圳市儿童医院院级课题(ynkt2020-zz19)。

摘　　要：目的探讨酿脓链球菌,即β溶血性A族链球菌(GAS)在深圳的流行病学特征。方法对2016年1月至2018年12月深圳市儿童医院分离的GAS流行克隆群进行多基因序列分型(MLST)回顾性分析。本研究中32株分属于7个不同emm分型的GAS菌株分别来自32例患有脓疱病、蜂窝组织炎、猩红热、脓毒症、肺炎、阻塞性睡眠呼吸暂停低通气综合征、支气管炎、过敏性鼻炎、臀部脓肿、过敏性紫癜或咽扁桃体炎的患儿,分离自咽拭子23份,痰液5份,脓液3份和血液1份。应用聚合酶链反应技术对32株GAS菌株的7对等位看家基因(gki、gtr、murI、mutS、recP、xpt和yqiL)进行扩增,对目标基因产物进行测序。然后将获得的每个等位基因的基因序列提交至MLST网站,以获得相应的等位基因谱。最后将等位基因谱再次提交至MLST网站,以确认序列分型(ST)。结果emm 1.00及其亚型、emm 4.00、emm 12.00及其亚型、emm 22.00、emm 28.00、emm 75.00和emm 89.00的GAS克隆群分别属于MLST分型中的ST28、ST39、ST36、ST46、ST52、ST49和ST921。结论2016年至2018年导致深圳地区儿童感染的GAS分离株的MLST流行克隆群分别为ST28、ST39、ST36、ST46、ST52、ST49和ST921。Objective To explore the epidemiological characteristics of Streptococcus pyogenes,namelyβ-hemolytic Group A Streptococcus(GAS)in children in Shenzhen.Methods Multilocus sequence typing(MLST)data on the epidemic clonal population of GAS infection in children in Shenzhen Children′s Hospital from January 2016 to December 2018 were retrospectively analyzed.In the present study,32 GAS strains belonging to 7 different emm types were from 32 children′s with impetigo,cellulitis,scarlet fever,sepsis,pneumonia,obstructive sleep apnea hypopnea syndrome,bronchitis,allergy with rhinitis,buttock abscess,allergic purpura or pharyngeal tonsillitis,which were isolated from 23 throat swabs,5 sputum samples,3 pus and 1 blood.Using polymerase chain reaction technology,7 pairs of allelic housekeeping genes(gki,gtr,murI,mutS,recP,xpt and yqiL)of 32 GAS isolates were analyzed,and the target gene products were subjected to sequencing.Then the obtained gene sequences of each allele were submitted to the MLST database to obtain the allele profile.Finally,the allele profiles were introduced in the MLST database again to confirm the sequence typing(ST).Results The GAS clone groups of emm 1.00 and its subtypes,emm 4.00,emm 12.00 and its subtypes,emm 22.00,emm 28.00,emm 75.00,and emm 89.00 belonged to the sequence typing ST28,ST39,ST36,ST46,ST52,ST49,and ST921,respectively.Conclusions From 2016 to 2018,the MLST clone populations of GAS isolates causing infections in children in Shenzhen are classified as ST28,ST39,ST36,ST46,ST52,ST49 and ST921.

关 键 词：酿脓链球菌 多基因序列分型 emm分型 儿童 

分 类 号：R446.5[医药卫生—诊断学]