西双版纳与海南橡胶林根际细菌群落组成与多样性研究  

Rhizosphere Microbial Composition of Hevea brasiliensis Plantations in Xishuangbanna and Hainan

作　　者：杜昊楠 魏亚情 全飞 陈伟[1] 兰国玉[2,3] 吴志祥 DU Hao-nan;WEI Ya-qing;QUAN Fei;CHEN Wei;LAN Guo-yu;WU Zhi-xiang(School of Tropical Crops,Hainan University,Haikou Hainan 570228,P.R.China;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou Hainan 571101,P.R.China;Danzhou Investigation&Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs,Danzhou Hainan 571737,P.R.China)

机构地区：[1]海南大学热带作物学院,海南海口570228 [2]中国热带农业科学院橡胶研究所,海南海口571101 [3]农业农村部儋州热带作物科学观测实验站,海南儋州571737

出　　处：《西部林业科学》2022年第1期77-83,共7页Journal of West China Forestry Science

基　　金：海南省自然科学基金高层次人才项目(320RC733);海南省重点研发项目社会发展方向项目(ZDYF2019145);国家自然科学基金(31770661);现代农业产业技术体系建设专项资金(CARS-33-ZP3)。

摘　　要：微生物在推动营养物质在土壤的生物化学循环、促进植物生长和抑制植物病害中起着非常重要的作用。为了解西双版纳地区和海南地区橡胶林根际微生物群落种类组成和空间分布对多样性格局特征的影响,采用高通量测序的方法,在西双版纳地区和海南地区分别选取3个地区橡胶林作为研究对象,分析根际细菌的组成多样性和大尺度地理分布格局。结果显示:橡胶树根际土壤细菌群落组成和多样性在大尺度地理位置上存在明显差异,PCoA分析西双版纳与海南的地理位置因素对组间差异的解释度为18.15%,Venn图显示163属是西双版纳植胶区特有的,占所有菌属种类的16.86%。Shannon指数西双版纳植胶地区显著高于海南植胶区。西双版纳地区根际独有属的比例较高。土壤有机质(SOM)、全磷(TP)、全钾(TK)是显著影响根际细菌群落大尺度地理分布最主要因子。In order to understand the effects of species composition and spatial distribution of microbial communities in the rhizosphere of Hevea brasiliensis forests in Xishuangbanna and Hainan on the characteristics of diversity pattern, the method of high-throughput sequencing was used in this paper.Three rubber plantations in Xishuangbanna and Hainan were selected as the research objects, and the composition diversity and large-scale geographical distribution pattern of rhizosphere bacteria were analyzed.The results showed that there were significant differences in the composition and diversity of bacterial communities in the rhizosphere of rubber trees on a large scale.PCoA analysis of geographical location factors in Xishuangbanna and Hainan explained the differences between groups with a degree of 18.15%.Venn diagram showed that 163 genera were endemic to Xishuangbanna H.brasiliensis planting area, accounting for 16.86% of all bacterial species.The Shannon index in Xishuangbanna H.brasiliensis planting area was significantly higher than that in Hainan rubber planting area.The proportion of exclusive genera in the rhizosphere of Xishuangbanna is high.Soil organic matter(SOM),total phosphorus(TP)and total potassium(TK)are the main factors that significantly affect the large-scale geographical distribution of rhizosphere bacteria.

关 键 词：橡胶林 根际细菌 多样性 西双版纳 海南 

分 类 号：S714.3[农业科学—林学]