不同干燥方式对猴头菌子实体多酚提取效果及其抗氧化活性的影响  

Different Drying Methods Affected Extraction and Antioxidant Activity of Polyphenols in Hericium erinaceus Fruiting Bodies

作　　者：杜笑 郭杨昪 常明昌[1,2,3] 孟俊龙 邓冰[1,2,3] 刘靖宇 DU Xiao;GUO Yangbian;CHANG Mingchang;MENG Junlong;DENG Bing;LIU Jingyu(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China;Shanxi Key Laboratory of Edible Fungi for Loess Plateau,Jinzhong 030801,Shanxi,China;Collaborative Innovation Center for Improving Quality and Efficiency of Edible Fungi on the Loess Plateau,Jinzhong 030801,Shanxi,China)

机构地区：[1]山西农业大学食品科学与工程学院,山西晋中030801 [2]黄土高原食用菌山西省重点实验室,山西晋中030801 [3]黄土高原食用菌提质增效协同创新中心,山西晋中030801

出　　处：《食用菌学报》2022年第2期65-72,共8页Acta Edulis Fungi

基　　金：国家自然科学基金(32072647);山西省应用基础研究项目(201801D121240);食用菌山西省科技创新重点团队(201805D131009)。

摘　　要：研究热风(60℃)、远红外辐射(50℃、48 h)和真空冷冻(-20℃、12 h,-80℃、12 h,-80℃、1 kPa、48 h)3种干燥方式对猴头菌(Hericium erinaceus)子实体多酚提取效果、体外抗氧化和细胞水平抗氧化活性的影响。结果表明:真空冷冻干燥处理组的游离酚、结合酚的提取得率(0.319%、0.035%)和纯度(20.784%、87.165%)较高。在3种干燥处理组中,游离酚对DPPH自由基的清除率和总抗氧化能力均以热风处理组最佳,而不同处理组的结合酚对DPPH自由基的清除率差异不显著,其总抗氧化能力则以热风处理组最佳;与体外抗氧化检测结果不同,猴头菌子实体游离酚与结合酚对H;O;诱导RAW264.7细胞氧化损伤的修复效果均以真空冷冻处理组最佳。Three drying methods, hot air drying(H, 60 ℃), far-infrared radiation drying(I, 50 ℃ for 48 h) and vacuum freeze drying(F,-20 ℃ for 12 h,-80 ℃ for 12 h,-80 ℃ and 1 kPa for 48 h), were studied for their effects on extraction and antioxidant activity of polyphenols in Hericium erinaceus fruiting bodies(HEFs). The results showed that vacuum freeze drying resulted in better extraction rates of both free(0.319%) and bound(0.035%) polyphenols with a higher purity(free polyphenols 20.784%, bound polyphenols 87.165%) than the other two drying treatments. Among the three drying treatments, H treatment resulted in the greatest DPPH scavenging rate and the strongest total antioxidant capacity of free polyphenols. There was no significant difference between different treatments on DPPH scavenging rate of bound polyphenols. H treatment showed a stronger total antioxidant capacity of bound polyphenols than the other two treatments. On the other hand, both free and bound polyphenols of fruiting bodies treated with F showed the best reparative effect on H;O;-induced oxidative damage to RAW264.7 macrophages.

关 键 词：干燥方式 猴头菌子实体 游离酚 结合酚 抗氧化 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]