基于SWATH蛋白定量技术研究溃疡性结肠炎脾虚湿蕴证的差异表达蛋白  

Study on differentially expression protein of ulcerative colitis patients with spleen deficiency and dampness stagnancy syndrome based on SWATH protein quantitative technology

作　　者：陈一鸣 胡月[1] 侯江涛[2] 侯政昆[2] 陶双友[2] 兰绍阳[2] 蔡佳仲[3] 张诗静 彭彬 文艺[2] 陈新林[1] CHEN Yi-ming;HU Yue;HOU Jiang-tao;HOU Zheng-kun;TAO Shuang-you;LAN Shao-yang;CAI Jia-zhong;ZHANG Shi-jing;PENG Bin;WEN Yi;CHEN Xin-lin(School of Basic Medical Science,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;No.1 Affiliated Hospital of Guangzhou University of Chinese Medicine,First School of Clinic Medicine,Guangzhou 510400,China;Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 510400,China)

机构地区：[1]广州中医药大学基础医学院,广州510006 [2]广州中医药大学第一附属医院第一临床医学院,广州510400 [3]广州中医药大学科技创新中心,广州510400

出　　处：《中华中医药杂志》2022年第2期698-703,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81774451,No.81703793);广东省自然科学基金项目(No.2017A030313827);广州中医药大学“薪火计划”资助项目(No.XH20190102)。

摘　　要：目的:筛选溃疡性结肠炎(UC)脾虚湿蕴证的差异表达蛋白,为进一步研究病证机制提供物质基础。方法:纳入UC脾虚湿蕴证患者4例和对照人群7例,采集受试者的肠黏膜组织,采用SWATH蛋白定量技术对蛋白进行定量鉴定,|log2 fold-change|>1,而且调整P值(FDR)≤0.05的蛋白质被认为是差异表达蛋白,并对差异表达蛋白进行基因本体、KEGG通路、蛋白互作用网络等生物信息学分析。结果:UC脾虚湿蕴组与对照组比较,存在差异表达的蛋白质有55种,表达上调的差异蛋白43种,表达下调的差异蛋白12种。差异蛋白主要参与23种生物过程、13种细胞组分和9种分子功能,补体C3(C3)、血浆激肽释放酶(KLKB1)、肝素辅因子2(SERPIND1)、补体成分C8α链(C8A)、CD59糖蛋白(CD59)、整合素α-X(ITGAX)显著富集于凝血与补体级联反应信号通路。结论:UC脾虚湿蕴证的病理机制可能和凝血与补体级联反应信号通路有关。Objective: To screen differentially expression proteins(DEPs) of ulcerative colitis(UC) patients with spleen deficiency and dampness stagnancy syndrome, and providing evidence for exploring possible mechanism of disease and syndrome.Methods: Biopsies of the colon mucosa were collected from 4 UC patients with spleen deficiency and dampness stagnancy syndrome and 7 health controls without any intestinal disease. The samples of all the subjects were studied with SWATH(sequential window acquisition of all theoretical spectra) method. Proteins with a |log2 fold-change|>1 and an adjusted P-value(FDR)<0.05 were considered to be significantly differentially expressed. Further bioinformatics analysis to classify the proteins in the gene ontology items and pathways. Results: Based on the quantified results, 55 differentially expressed proteins were identified,including 43 up-regulated proteins and 12 down-regulated proteins. Differentially expressed proteins are mainly involved in 23 biological processes, 13 cell components and 9 molecular functions. Complement C3, plasma kallikrein, heparin cofactor 2,complement component C8 alpha chain, CD59 glycoprotein and integrin alpha-X were significantly enriched in complement and coagulation cascades signal pathway. Conclusion: The pathogenesis of UC patients with DSSD may be achieved through coagulation and complement cascade’s reaction signal pathway.

关 键 词：溃疡性结肠炎 蛋白质组学 脾虚湿蕴证 SWATH蛋白定量技术 

分 类 号：R259[医药卫生—中西医结合]