磷脂酶A2受体基因多态性与特发性膜性肾病气阴两虚证的相关性研究  

Research on the correlation between phospholipase A2 receptor gene polymorphism and qi-yin deficiency syndrome of idiopathic membranous nephropathy

作　　者：彭贵军[1] 胡影影 吴迪 PENG Gui-jun;HU Ying-ying;WU Di(The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450008,China;The Ninth People's Hospital of Zhengzhou,Zhengzhou 450000,China;Henan Province Hospital of TCM,Zhengzhou 450000,China)

机构地区：[1]河南中医药大学第一附属医院,郑州450008 [2]郑州市第九人民医院,郑州450000 [3]河南省中医院,郑州450000

出　　处：《中华中医药杂志》2022年第2期1145-1148,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南省中医药研究专项课题(No.2017JDZX024)。

摘　　要：目的:探讨磷脂酶A2受体(PLA2R)相关基因位点(rs35771982、rs3828323)与特发性膜性肾病(IMN)气阴两虚证的相关性。方法:将IMN患者66例分为气阴两虚证、非气阴两虚证两组,每组随机抽取15例患者外周血进行PLA2R SNP rs35771982、rs3828323基因型分析、基因突变位点序列、峰图位点测序等检测。结果:在两组患者中两基因位点基因型及其等位基因频率分布差异均有统计学意义(P<0.05),即PLA2R SNP rs35771982位点CC基因型、C等位基因较CG基因型、G等位基因构成比高,PLA2R SNP rs3828323位点CC基因型、C等位基因较CT基因型、T等位基因构成比高。结论:PLA2R基因多态性与IMN中医证型之间存在联系,PLA2R SNP rs3828323位点CC基因型和C等位基因可能与IMN多表现为气阴两虚证密切相关。Objective: To investigate the correlation between phospholipase A2 receptor(PLA2R) related gene loci(rs35771982, rs3828323) and idiopathic membranous nephropathy(IMN) in qi-yin deficiency syndrome. Methods: A total of 66IMN patients were divided into two groups: qi-yin deficiency syndrome and without qi-yin deficiency syndrome. Fifteen peripheral blood samples were collected from each group respectively and sent to the genomics institute for genotype analysis of PLA2R SNP rs35771982 and rs3828323, gene mutation site sequence and peak map site sequencing and so on. Results: Genotypes and alleles frequency distribution of two gene loci had statistical significance in the two groups(P<0.05), namely PLA2R SNP rs35771982 site CC genotype and C allele had higher composition ratio than CG genotype and G allele, and PLA2R SNP rs3828323 site CC genotype and C allele had higher composition ratio than CT genotype and T allele. Conclusion: There was an association between PLA2R gene polymorphism and TCM syndromes of IMN. CC genotype and C allele at site of PLA2R SNP rs3828323 may be closely related to the deficiency of both qi and yin in IMN.

关 键 词：磷脂酶A2受体 基因多态性 特发性膜性肾病 中医证型 

分 类 号：R277.5[医药卫生—中医学]