苋菜MDH基因克隆及生物信息学分析  

Clone and Bioinformatics Analysis of MDH Gene in Amaranthus tricolor

作　　者：纪雅婷 赵梅 陈家兰 郑友峰 赖钟雄[1] 刘生财[1] JI Ya-ting(Institute of Horticultural Plant Bioengineering,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《园艺与种苗》2022年第2期6-8,52,共4页Horticulture & Seed

基　　金：福建农林大学大学生创新创业项目(111ZC0010);福建农林大学经济作物种业服务团队(11899170125)。

摘　　要：[目的]对苋菜MDH基因进行克隆及生物信息学分析。[方法]以苋菜试管苗为材料,从实验室构建的苋菜转录组数据库中筛选出苋菜MDH基因的c DNA序列片段,采用RT-PCR技术对其ORF进行克隆,并进行生物信息学分析。[结果]MDH基因编码344个氨基酸,通过生物信息学分析表明,MDH可能为非分泌蛋白,包括33个磷酸化位点,二级结构以α螺旋为主,三级结构是由无规则卷曲结构、α螺旋和β折叠构成的,与藜麦有较近的进化关系,在黑暗条件下和叶片红色部位表达更显著开花期较其他生长时期表达更显著。[结论]为将来深入研究MDH基因功能奠定基础。[Objective]To clone and bioinformatics of MDH gene in Amaranthus tricolor.[Method]Using amaranth test-tube seedlings as material,from the amaranth transcriptome database constructed in the laboratory,the cDNA sequence fragment of the MDH gene of amaranth was screened,and its ORF was cloned by RT-PCR and analyzed by bioinformatics.[Result]The results show that the MDH gene encodes 344 amino acids.Bioinformatics analysis shows that MDH may be a non-secreted protein,including 33 phosphorylation sites.The secondary structure is dominated by α helix,and the tertiary structure is composed of random coiled structures,Α-helix and β-sheet,it has a close evolutionary relationship with quinoa.[Conclusion]The expression is more significant in dark conditions and red parts of leaves,and more significant in flowering stage than in other growth stages.

关 键 词：苋菜 苹果酸脱氢酶(MDH) 开放阅读框 克隆 生物信息学分析 

分 类 号：Q81[生物学—生物工程] S636.4[农业科学—蔬菜学]