棘孢木霉分泌蛋白和效应子的预测及分析  

Prediction and analysis of sereted proteins and effectors of Trichoderma asperellum

作　　者：李枭艺 董章勇 罗梅[1,2] LI Xiaoyi;DONG Zhangyong;LUO Mei(College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Innovative Institute of Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China)

机构地区：[1]仲恺农业工程学院农业与生物学院,广东广州510225 [2]仲恺农业工程学院植物健康创新研究院,广东广州510225

出　　处：《仲恺农业工程学院学报》2022年第1期13-17,共5页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省普通高校特色创新类项目(2018KTSCX097)资助.

摘　　要：棘孢木霉(Trichoderma asperellum)作为一种生防菌,在植物病害的防控中具备较高的开发以及利用价值.根据棘孢木霉CBS 433.97菌株全基因组测序获得的12557个蛋白序列,利用SignalP、TargetP、TMHMM、WoLF PSORT和big-PI Predictor生物信息学软件和预测程序依次进行筛选,最终获得485个具有典型特征的分泌蛋白序列.进一步将485个分泌蛋白与碳水化合物活性酶数据库进行比对、半胱氨酸含量分析、与病原物-寄主互作数据库比对终获得137个蛋白;进一步筛选<300氨基酸的小分子蛋白作为候选效应蛋白,最终获得15个蛋白,其中1个为碳水化合物结合模块家族蛋白,1个为碳水化合物酯酶蛋白,其余13个均为假定蛋白.研究结果为进一步研究棘孢木霉基因的作用机理及其后续的改造利用提供了一定的参考.Trichoderma asperellum as a biocontrol strain has high development and application value in plant pathologic diseases.According to the 12557 proteins in the genome of T.asperellum CBS 433.97 strain,it was in order screened by SignalP,TargetP,TMHMM,WoLF PSORT and big-PI Predictor bioinformatics softwares and prediction programs.Finally,485 secreted protein sequences with typical characteristics were obtained.A total of 137 proteins were obtained by comparing 485 secreted proteins with the carbohydrate-active enzymes database,cysteine content analysis and Pathogen-Host Interaction database analysis.The small molecule protein of less than 300 amino acid was further screened as the candidate effector protein,and 15 proteins were obtained,including one carbohydrate-binding module family protein,one carbohydrate-esterase protein,and 13 hypothetical proteins.The results provide a theoretical basis for further study on the mechanism of action of T.asperellum genes and its subsequent utilization.

关 键 词：棘孢木霉 全基因组 分泌蛋白 

分 类 号：S432.1[农业科学—植物病理学]