实验用巴马小型猪行硬脑膜成形术全身麻醉的实施及管理  

Administration and management of general anesthesia in Bama minipigs undergoing duraplasty

作　　者：钟红亮[1] 王振民[2] 范议方[3] 刘海洋[3] 汪阳 刘丕楠[2] ZHONG Hong-liang;WANG Zhen-min;FAN Yi-fang;LIU Hai-yang;WANG Yang;LIU Pi-nan(Department of Neurosurgery,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China;Department of Neurosurgery,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China;Department of Anesthesiology,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China)

机构地区：[1]首都医科大学附属北京朝阳医院神经外科,北京100020 [2]首都医科大学附属北京天坛医院神经外科,北京100070 [3]首都医科大学附属北京天坛医院麻醉科,北京100070

出　　处：《中国临床神经外科杂志》2022年第3期178-181,共4页Chinese Journal of Clinical Neurosurgery

基　　金：国家自然科学基金(30870752)。

摘　　要：目的探讨实验用巴马小型猪行硬脑膜成形术时全身麻醉的实施及管理的方法。方法将12头巴马小型猪按随机数字表法随机分为观察组(n=6)和对照组(n=6)。对照组采用传统的纤维蛋白胶粘合法进行硬脑膜成形术,观察组采用新的CO_(2)激光焊接法进行硬脑膜成形术。麻醉方法:耳后肌肉注射氯胺酮(10 mg/kg)、咪达唑仑(0.5 mg/kg)和阿托品(0.5 mg)混合液进行基础麻醉,耳缘静脉给予0.05 mg芬太尼和罗库溴铵(0.6 mg/kg)进行诱导麻醉,持续吸入1.5%~2%的异氟烷维持麻醉。麻醉即刻、10 min、30 min、1 h、2 h、3 h、4 h,记录两组小型猪血压(BP)、心率(HR)、呼吸频率(RR);持续监测血氧饱和度。记录麻醉诱导开始至意识消失时间、停止吸入麻醉至自主呼吸恢复时间、至清醒时间;记录术后并发症。结果全麻维持效果Ⅰ级11只;Ⅱ级1只(对照组);脉搏血氧饱和度维持在95%~100%;麻醉后1 h,两组小型猪HR、RR和BP下降并维持在稳定水平,两组均无统计学差异(P>0.05)。两组巴马小型猪麻醉诱导时间、停止吸入麻醉至自主呼吸恢复时间、停止吸入麻醉至清醒时间均无统计学差异(P>0.05)。12头巴马小型猪全部存活,无麻醉相关并发症。结论应用巴马小型猪进行脑膜修补术时,应用芬太尼联合罗库溴铵行静脉诱导、术中持续吸入异氟烷进行维持麻醉是安全、可行的。Objective To investigate the method of general anesthesia in Bama minipigs undergoing duraplasty. Methods Twelve Bama minipigs were randomly divided into observation group(n=6) and control group(n=6). The control group was treated with traditional fibrin glue bonding method for duraplasty, while the observation group was treated with new CO2laser welding method for duraplasty. Anesthesia method: basal anesthesia was performed by intramuscular injection of ketamine(10 mg/kg), midazolam(0.5 mg/kg) and atropine(0.5 mg) behind the ear, and 0.05 mg of fentanyl and rocuronium(0.6 mg/kg) were given to the marginal ear vein for induction of anesthesia, and anesthesia was maintained with continuous inhalation of 1.5%~2% isoflurane. Immediately after anesthesia,10 min, 30 min, 1 h, 2 h, 3 h, and 4 h, the blood pressure(BP), heart rate(HR), and respiratory rate(RR) of the two groups were recorded;and blood oxygen saturation was continuously monitored. The time from the start of anesthesia induction to the disappearance of consciousness, the time from the stop of inhalation anesthesia to the recovery of spontaneous breathing, and the time to awake were recorded. Postoperative complications were recorded. Results General anesthesia of grade Ⅰ was maintained in 11 pigs, grade Ⅱ in 1(control group). The pulse oxygen saturation was maintained at 95%~100%. One hour after the anesthesia, the HR, RR and BP of the two groups decreased and remained stable, and there was no statistical difference between the two groups(P>0.05). There were no significant differences in the time of induction of anesthesia, the time from stopping inhalation anesthesia to spontaneous breathing recovery, and the time from stopping inhalation anesthesia to awake between the two groups(P>0.05). All 12 Bama minipigs survived without complications related to anesthesia. Conclusions It is safe and feasible to use fentanyl combined with rocuronium bromide for intravenous induction and continuous inhalation of isoflurane to maintain anesthesia during duraplas

关 键 词：小型猪 硬脑膜成形术 全身麻醉 

分 类 号：R651.11[医药卫生—外科学] R614[医药卫生—临床医学]