生物催化氨基酸C—N裂解反应的研究进展  

Advances in biocatalytic C-N cleavage of amino acids

作　　者：武耀运 陈城虎 宋伟 胡贵鹏 刘佳[1] 吴静 WU Yaoyun;CHEN Chenghu;SONG Wei;HU Guipeng;LIU Jia;WU Jing(State Key Laboratory of Food Science&Technology,Jiangnan University,Wuxi 214122,China;School of Life Sciences and Health Engineering,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学生命科学与健康工程学院,江苏无锡214122

出　　处：《生物加工过程》2022年第2期137-147,共11页Chinese Journal of Bioprocess Engineering

基　　金：国家重点研发计划(2021YFC2100100);国家自然科学基金青年基金(22008089、21878146);江苏省自然科学基金青年基金(BK20200622)。

摘　　要：氨基酸脱氨酶能催化系列氨基酸C—N裂解反应生成对应的α-酮酸和氨,是代谢途径及生物催化的重要酶。综述了近年来催化氨基酸C—N裂解反应的5’-磷酸吡哆醛介导的苏氨酸脱氨酶、黄素腺嘌呤二核苷酸介导的L-氨基酸脱氨酶和L-氨基酸氧化酶,以及这些关键酶应用于多酶级联反应中以生产α-羟基酸、α-酮酸、D-氨基酸等精细化学品的研究进展。此外,还对该类反应的应用前景进行了展望,以期扩大其在精细化学品生产中的应用范围。Amino acid deaminase is an important enzyme in metabolic pathway and biocatalysis to catalyze C-N cleavage of a series of amino acids to produce corresponding α-keto acids and ammonia.This review introduces the key enzyme preparations such as 5’-pyridoxal phosphate mediated threonine deaminase, flavin adenine dinucleotide mediated L-amino acid deaminase and L-amino acid oxidase that catalyze this reaction and application in multi-enzyme production of fine chemicals such as L-aminobutyric acid, α-hydroxy acids, α-keto acids, D-amino acids and their derivatives, R/S-hydroxy acids and aromatic alcohols.Furthermore, the application prospect is addressed, so as to expand its application range in fine chemicals production.

关 键 词：氨基酸脱氨酶 C—N裂解反应 生物催化 多酶级联反应 精细化学品 

分 类 号：Q554[生物学—生物化学]