不同酶联免疫吸附法检测丙肝抗体的效果  

Effects of different enzyme-linked immunosorbent assays in detecting hepatitis C antibody

作　　者：吴菲菲[1] Wu Feifei(Department of Microorganism Detection,Luohu District Center for Disease Prevention and Control,Shenzhen 518020,China)

机构地区：[1]深圳市罗湖区疾病预防控制中心微检科,深圳518020

出　　处：《国际医药卫生导报》2022年第7期946-949,共4页International Medicine and Health Guidance News

基　　金：深圳市罗湖区科技局立项(LX20211105)。

摘　　要：目的研究用不同酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测丙型肝炎(简称丙肝)抗体的效果。方法选取深圳市罗湖区辖区医院2019年6月至2021年6月收治的20~65岁男性80例丙肝患者血清,根据检测方式分为常规组(间接ELISA法,40例)和试验组血清(双抗原夹心ELISA法,40例),比较分析两组检测结果。采用独立样本t检验和χ^(2)检验。结果抗-丙型病毒性肝炎(viral hepatitis C,HCV)阳性标本有8份,试验组阳性检出率为87.50%(7/8),常规组阳性检出率为25.00%(2/8);抗-HCV阴性标本有72份,试验组阴性检出率为97.22%(70/72),常规组阴性检出率为83.33%(60/72);两组比较,差异均有统计学意义(χ^(2)=6.3492、7.9121,P=0.0117、0.0049)。试验组灵敏度、特异度、阳性预测值及阴性预测值均高于常规组,差异均有统计学意义(均P<0.05)。结论双抗原夹心ELISA法检测丙肝抗体具有较高的特异度、灵敏度,可为疾病治疗提供参考数据,达到改善预后作用,值得推广。Objective To study the effects of different enzyme-linked immunosorbent assays(ELISA)in detecting hepatitis C antibody.Methods Eighty male hepatitis C patients who were 20-65 years old and who were admitted to hospitals at Luohu District from June 2019 to June 2021 were selected.According to the detection methods,they were divided into a conventional group(40 cases),detected by the indirect ELISA,and an experimental group(40 cases),detected by the double antigen sandwich ELISA.The test results of the two groups were compared and analyzed.The independent-sample t and χ^(2) test were applied.Results There were 8 anti-viral hepatitis C(HCV)positive samples,and the positive detection rate was 87.50%(7/8)in the experimental group,and was 25.00%(2/8)in the conventional group;there were 72 anti-HCV negative samples,and the negative detection rate was 97.22%(70/72)in the experimental group,and was 83.33%(60/72)in the conventional group;there were statistical differences between these two groups(χ^(2)=6.3492 and 7.9121;P=0.0117 and 0.0049).The sensitivity,specificity,and positive and negative predictive values in the experimental group were higher than those in the conventional group,with statistical differences(all P<0.05).Conclusion The double antigen sandwich ELISA has high specificity and sensitivity in the detection of hepatitis C antibody,so it can provide reference data for disease treatment and prognosis improvement,and is worth being generalized.

关 键 词：ELISA实验 丙肝抗体 诊断结果 诊断效能 

分 类 号：R446.6[医药卫生—诊断学] R512.63[医药卫生—临床医学]