胰蛋白酶水解羊血清蛋白工艺研究  

Technology Researches on Serum Protein of Tibetan Sheepwith Trypsin Hydrolysis Method

作　　者：张娅俐 洪晶 张棚 田晓静[1,2] 曹竑 丁功涛[1,2] 罗丽 ZHANG Ya-li;HONG Jing;ZHANG Peng;TIAN Xiao-jing;CAO Hong;DING Gong-tao;LUO Li(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730124,China;China-Malaysia National Joint Laboratory,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China;Gannan Institute of Yak Milk,Hezuo 747000,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]西北民族大学生物医学研究中心中国-马来西亚国家联合实验室,甘肃兰州730030 [3]甘南牦牛乳研究院,甘肃合作747000

出　　处：《西北民族大学学报（自然科学版）》2022年第1期40-46,共7页Journal of Northwest Minzu University(Natural Science)

基　　金：中央高校基本科研业务费专项资金项目(31920200115);科技部援助项目(KY201501005);甘肃省自然科学基金项目(18JR3RA371)。

摘　　要：为进一步利用血液资源,以羊血清蛋白为原料来探究胰蛋白酶水解羊血清蛋白的工艺.以水解度为指标,试验采用单因素研究pH、温度、加酶量和水解时间四个因素对水解度的影响.在此基础上,采用Box-Benhnken响应面试验设计优化胰蛋白酶水解藏系绵羊血清蛋白的工艺条件.结果表明:在底物浓度为5%,加酶量为9000 U/g时,最佳水解条件为:反应时间5.6 h、温度55℃、pH10.0,在此条件下水解度可达到19.32±0.21%.In order to make further use of blood resources,the process of pantetic protease hydrolysis of amniotic serum protein is explored with amniotic serum protein as raw material.Taking hydrolysis degree as the index,the single factor design was used in the present experiment so as to study the effects of pH,temperature,enzyme amount and hydrolysis time on hydrolysis of serum protein.On this basis,the process conditions of panteinase hydrolysis serum protein in sheep were optimized by Box-Benhnken response surface test.The results showed that when the substrate concentration was 5%and the enzymatic amount was 9000 U/g,the optimum hydrolysis conditions were:reaction time was 5.6 h,temperature was 55℃,pH was 10.0,and hydrolysis could reach 19.32±0.21%under this condition.

关 键 词：胰蛋白酶 藏系绵羊 水解血清蛋白 响应面分析 

分 类 号：Q819[生物学—生物工程]