芦根提取物对大鼠急性酒精性肝损伤的保护作用研究  

Study on the Protective Effect of Rhizoma Phragmitis Extracts on Acute Alcoholic Liver Injury in Rats

作　　者：饶智 陈光宇[1,2,3] 谢梦洲 陈芳 陈俣祯[1,2,3] 蒋星 田慧 李雅云 RAO Zhi;CHEN Guang-yu;XIE Meng-zhou;CHEN Fang;CHEN Yu-zhen;JIANG Xing;TIAN Hui;LI Ya-yun(Hunan University of Chinese Medicine,Changsha 410208,China;Engineering Center of Medicinal and Edible Functional Food,Hunan University of Chinese Medicine,Changsha 410208,China;Key Laboratory of Cardiopulmonary Disease Syndrome Differentiation and Medicinal Diet Therapy in Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省药食同源功能性食品工程技术研究中心,湖南长沙410208 [3]中医心肺病证辨证与药膳食疗重点研究室,湖南长沙410208 [4]湖南中医药大学中医学院,湖南长沙410208

出　　处：《时珍国医国药》2022年第1期95-98,共4页Lishizhen Medicine and Materia Medica Research

基　　金：湖南省教育厅科学研究基金(18B237);湖南中医药大学药食同源功能性食品工程技术研究中心开放基金(2018YSTY02)。

摘　　要：目的探究芦根中各提取成分对大鼠急性酒精性肝损伤的保护作用。方法大鼠被随机分为空白组、模型组、阳性组、芦根多糖组、芦根果胶组、芦根提取物A组,每组9只,共54只。各组大鼠分别预防性给药14天后,除空白组外,其余各组给予体积分数50%的乙醇14ml/(kg BW)建立急性酒精性肝损伤模型。16h后测定大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)、甘油三酯(TG)、极低密度脂蛋白(VLDL)含量,苏木精-伊红染色观察大鼠肝组织病理学变化。结果与模型组相比,芦根多糖组、芦根果胶组、芦根提取物A组大鼠血清中ALT、AST、LDH、TG、VLDL含量均明显下降,差异具有统计学意义(除芦根多糖组、芦根果胶组大鼠血清中AST含量和模型组相比较P>0.05,其余均为P<0.05或P<0.01),并能改善肝脏病理学变化情况。结论芦根多糖、芦根果胶和芦根提取物A对大鼠急性酒精性肝损伤具有明显的保护作用,作用机制可能与其抗氧化和改善肝脏脂质代谢功能有关。Objective To investigate the protective effect of various extracts from Rhizoma Phragmitis on acute alcoholic hepatic injury in rats.Methods Rats were randomly divided into 6 groups(blank control,model control,drug control,RP polysaccharide group,RP pectin group,RP extract group A),with 9 rats in each group and a total of 54 rats.After prophylactic administration for 14 days,the rats in each group were given 14 mL/(kg BW)of ethanol with 50% volume fraction to establish the model of acute alcoholic liver injury,except for the blank group.After 16 h,the contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH),triglyceride(TG)and very low density lipoprotein(VLDL)in serum of rats were determined.Hematoxylin-eosin(HE)staining was performed to observe the pathological changes of liver tissues.Results Compared with the model group,the contents of ALT,AST,LDH,TG and VLDL in serum of rats in RP polysaccharide group,RP pectin group,RP extract group A were significantly decreased,and the differences were statistically significant(except for the contents of AST in RP polysaccharide group and RP pectin group,P>0.05,the rest were P<0.05 or P<0.01),and improved the pathological changes of liver tissues.Conclusion RP polysaccharide,RP pectin and RP extract A have protective effect on acute alcoholic liver injury in rats,and the mechanism of which may be related to its anti-oxidation and improvement of liver lipid metabolism.

关 键 词：芦根提取物 急性酒精性肝损伤 保护作用 抗氧化 脂质代谢 

分 类 号：R285.5[医药卫生—中药学]