补充维生素D对D-半乳糖诱导的衰老大鼠睾丸功能的改善作用及机制  

Vitamin D supplementation improves testicular function in aging rats induced by D-galactose

作　　者：刘丹青 崔颖 刘彦玲 郭丰[1] 杜培洁[1] 邵明玮[1] 刘艳霞[1] 秦贵军[1] Liu Danqing;Cui Ying;Liu Yanling;Guo Feng;Du Peijie;Shao Mingwei;Liu Yanxia;Qin Guijun(Department of Endocrine and Metabolic Diseases,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院内分泌科,450052

出　　处：《中华内分泌代谢杂志》2022年第2期151-155,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金(81770812,81974110)。

摘　　要：目的探讨补充维生素D对D-半乳糖诱导的衰老大鼠睾丸功能的作用及可能机制。方法利用皮下注射D-半乳糖诱导衰老大鼠模型,将实验动物随机分为6组:衰老(DG)组、衰老+低剂量维生素D补充(LD)组、衰老+高剂量维生素D补充(HD)组、正常对照组(NC)、正常+低剂量维生素D补充(LN)组、正常+高剂量维生素D补充(HN)组。测定各组大鼠体重、睾丸重量、血清睾酮浓度、精子质量。利用光镜检测睾丸形态学变化,利用分光光度计测定超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平,利用Western blot检测睾丸维生素D受体(VDR)、Nrf2、GCLC及SOD2的表达水平。`结果与NC组相比,DG组大鼠睾丸重量、血清睾酮水平、SOD活性、Nrf2、GCLC、SOD2表达水平显著降低,而MDA水平显著升高。经维生素D补充后,HD及LD组大鼠睾丸重量、睾酮水平及SOD活性显著升高,而MDA水平较前显著下降;Nrf2、GCLC、SOD2及VDR表达水平显著升高。结论维生素D可能通过激活Nrf2-ARE信号通路提高睾丸抗氧化应激能力,从而改善D-半乳糖诱导的衰老大鼠睾丸功能。Objective To investigate the effects and potential mechanism of vitamin D supplementation on testicular function in aging rats induced by D-galactose.Methods The aging rats were induced by D-galactose with subcutaneous injection.The animals were randomly divided into 6 groups:aging rats(DG),aging rats with low-dose vitamin D supplementation(LD),aging rats with high-dose vitamin D supplementation(HD),normal control rats(NC),normal rats with low-dose vitamin D supplementation(LN),normal rats with high-dose vitamin D supplementation(HN).The body weight,testicular weight,serum testosterone concentrations and sperm quality of the rats in each group were measured.The testis morphological changes were detected using light microscopy.The activity of superoxide dismutase(SOD)and level of malondialdehyde(MDA)were determined with spectrophotometer.The expression levels of Nrf2,GCLC,SOD2 and VDR in testis were detected by western blot.Results At baseline,compared with NC group,testicular weight,serum testosterone level,SOD activity,Nrf2,GCLC and SOD2 expression levels were significantly decreased in DG group,while MDA level was significantly increased.After vitamin D supplementation,testicular weight,testosterone levels and SOD activity in both of HD and LD groups were significantly increased,while the MDA level was significantly decreased.The expression levels of Nrf2,GCLC,SOD2 and VDR were significantly increased.Conclusion Vitamin D supplementation may enhance the testicular antioxidant capacity through activating Nrf2-ARE signaling pathway,and improve the testicular function in D-galactose-induced aging rats.

关 键 词：维生素D D-半乳糖 衰老 睾丸功能 Nrf2-ARE信号通路 

分 类 号：R698[医药卫生—泌尿科学]