大黄中总鞣质的提取方法研究  

Study on Extraction Process of Total Tannin in Rhubarb

作　　者：刘佳 黄思洋 陈钰沁 朱艳玲 赵明智 付娟姬 杨伶俐[3] LIU Jia;HUANG Siyang;CHEN Yuqin;ZHU Yanling;ZHAO Mingzhi;FU Juanji;YANG Lingli(Haiyuan College,Kunming Medical University,Kunming,Yunnan,China 650101;Kunming Pharmaceutical Group Xuesaitong Pharmaceutical Limited Liability Company,Wenshan,Yunnan,China 663400;Department of Science and Education,First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan,China 650000)

机构地区：[1]昆明医科大学海源学院,云南昆明650101 [2]昆药集团血塞通药业股份有限公司,云南文山663400 [3]昆明医科大学第一附属医院科教部,云南昆明650000

出　　处：《昆明学院学报》2021年第6期109-113,共5页Journal of Kunming University

基　　金：云南省教育厅科学研究基金资助项目(2021J0876)。

摘　　要：为建立大黄中总鞣质的最佳提取方法.采用超声波辅助提取法,并选取溶剂体积分数、提取时间和固液比3个主要因素,在单因素实验的基础上,通过正交实验,明确大黄中总鞣质的最佳提取方法.结果表明,当乙醇的体积分数为60%、提取时间为100 min、固液比为15倍时,提取率最高.说明利用超声波辅助法提取大黄中的总鞣质,操作简单、可行性较高.To establish the best extraction method of tannin from rhubarb, Ultrasonic-assisted extraction method was used. The three main elements as solvent volume fraction, the extraction time, and solid-liquid ratio are selected. On the basis of single factor experiment, through orthogonal experiment the best extraction method of total tannin from Rheum offcinale Baill. is determined. The results showed that when the volume fraction of ethanol was 60%, the extraction time was 100 min and the solid-liquid ratio was 15 times, the extraction yield was the highest, which states that ultrasonic assisted extraction of tannin from rhubarb is simple and feasible.

关 键 词：大黄 总鞣质 提取方法 单因素实验 正交实验 

分 类 号：O658[理学—分析化学]