骨关节炎患者膝关节滑膜CD1c^(+)髓源树突细胞的鉴定  

Identification of CD1c^(+) myeloid dendritic cells from the synovium of knee joint in patients with osteoarthritis

作　　者：邱国伟 肖涟波[1,2] 张晓明 孟璐[3] 钟声 解骏 孙松涛 QIU Guo-wei;XIAO Lian-bo;ZHANG Xiao-ming;MENG Lu;ZHONG Sheng;XIE Jun;SUN Song-tao(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Orthopedics,Guanghua Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200052,China;Institute Pasteur of Shanghai,Chinese Academy of Sciences,Shanghai 200053,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属光华医院矫形外科,上海200052 [3]中国科学院上海巴斯德研究所,上海200032

出　　处：《现代免疫学》2022年第1期31-36,共6页Current Immunology

基　　金：上海市十三五重点专科-中西医结合骨关节病科(shslczdzk04801);人工膝关节置换术围术期中西医结合快速康复(ZY-FWTX-6023);上海市第三批中医临床优势专科建设-骨伤科(ZYSNXD-YL-YSZK016)。

摘　　要：CD1c^(+)髓样树突细胞(myeloid dendritic cells,mDC)是一类重要的DC亚群,参与T细胞的免疫应答。为探讨膝骨关节炎患者关节滑膜是否存在CD1c^(+)mDC的分布,收集20例骨关节炎(osteoarthritis,OA)患者的外周血和膝关节滑膜,采用FACS检测外周血和关节滑膜内的CD1c^(+)mDC、单核/巨噬细胞和T细胞,免疫组化方法检测关节滑膜中CD1c、CD3、CD33分子的表达情况。结果显示,OA患者膝关节滑膜中的CD1c^(+)mDC和巨噬细胞的比例显著高于外周血(P<0.0001),而T细胞比例显著低于外周血(P<0.0001);关节滑膜中存在CD1c和CD3的弥漫性浸润且两者定位邻近。该研究提示,OA关节滑膜中存在一群比例显著高于外周血的CD1c^(+)mDC,CD1c^(+)mDC在骨关节炎发生、发展过程中具有重要意义。CD1c^(+)myeloid dendritic cells(mDC)are an important cluster of DCs,which participate in the immune response of T cells.To study whether there are CD1c^(+)mDC in the joint synovium of patients with knee osteoarthritis,the peripheral blood and knee synovium of 20 patients with osteoarthritis(OA)were collected.The CD1c^(+)mDCs,monocytes/macrophages,and T cells in these peripheral blood and knee synovium samples were detected with FACS.The expressions of CD1c,CD3,and CD33 in joint synovium were detected by immunohistochemistry.The results showed that the proportion of CD1c^(+)mDC and macrophages in the synovium of knee joint in OA patients was significantly higher than that in peripheral blood(P<0.0001),while the proportion of T cells was significantly lower than that in peripheral blood(P<0.0001).The infiltration of CD1c and CD3 in the synovium of knee joint was spatially adjacent.The above results suggest that a group of CD1c^(+)mDC cells is enriched in the synovium of OA joints and they might play an important role in the occurrence and development of osteoarthritis.

关 键 词：骨关节炎 关节滑膜 CD1c 树突状细胞 

分 类 号：R392.12[医药卫生—免疫学]