宫颈及宫内HPV感染与胎膜早破的相关性分析  

Analysis of the association between cervical and intrauterine infection with human papillomavirus(HPV)and premature rupture of the membranes

作　　者：吴金保[1] 李会俭 周杰[1] WU Jin-bao;LI Hui-jian;ZHOU Jie(Wuxi Maternal and Child Care Hospital,Wuxi,Jiangsu 214002,China)

机构地区：[1]无锡市妇幼保健院产科,江苏无锡214002

出　　处：《中国病原生物学杂志》2021年第12期1462-1464,共3页Journal of Pathogen Biology

摘　　要：目的探讨孕妇宫颈和宫内HPV感染与胎膜早破的关系。方法选取2017年1月-2019年1月本院收治的200例胎膜早破孕妇为研究对象,以同期200例正常孕妇为对照,采用荧光定量PCR技术对其宫颈分泌物及产后胎膜组织及羊水进行HPV检测并分型。比较胎膜早破与宫颈、宫内HPV感染的关系。结果200例胎膜早破孕妇组宫颈分泌物HPV阳性42例,阳性率21%。其中高危亚型32例,占76.19%;低危亚型10例,占23.81%。200例正常孕妇组中HPV阳性38例,阳性率19%。其中高危亚型30例,占78.95%;低危亚型8例,占21.05%。胎膜早破组与正常孕妇组比较HPV阳性率差异无统计学意义(χ^(2)=0.67,P>0.05);胎膜早破组HPV高危型与低危型占比差异有统计学意义(χ^(2)=29.17,P<0.01);正常孕妇组HPV高危型与低危型占比差异有统计学意义(χ^(2)=50.82,P<0.01)。200例胎膜早破孕妇胎膜组织及羊水HPV阳性28例,阳性率14%。其中高危亚型24例,占85.71%;低危亚型4例,占14.29%。200例正常孕妇组胎膜组织及羊水HPV阳性6例,阳性率3%,且均为高危亚型。胎膜早破孕妇组与正常孕妇组比较胎膜组织及羊水HPV阳性率差异有统计学意义(χ^(2)=34.30,P<0.01);胎膜早破孕妇组胎膜组织及羊水HPV高危型与低危亚型占比差异有统计学意义(χ^(2)=33.22,P<0.01)。结论妊娠期HPV感染以高危型为主,孕妇宫内感染HPV与胎膜早破显著相关,高危型HPV易发生宫内感染,引发孕妇胎膜早破。Objective To investigate the association between cervical and intrauterine infection with human papillomavirus(HPV)and premature rupture of the membranes among pregnant women.Methods Subjects were a total of 200 pregnant women with premature rupture of the membranes seen at Wuxi Maternal and Child Care Hospital from January 2017 to January 2019,and 200 women with a normal pregnancy seen at this Hospital during the same period served as controls.Cervical secretions and postpartum fetal membrane and amnionic fluid samples were collected for detection and genotyping of HPV using a fluorescent quantitative PCR(qPCR)assay.The association between premature rupture of the membranes and a cervical or intrauterine HPV infection was assessed.Results Cervical secretions from 200 pregnant women with premature rupture of the membranes tested positive for HPV at a rate of 21%,including 32 women(76.19%)with high-risk HPV and 10 women(23.81%)with low-risk HPV.Cervical secretions from 200 normal pregnant women tested positive for HPV at a rate of 19%,including 30 women(78.95%)with high-risk HPV and 8 women(21.05%)with low-risk HPV.There were no significant differences in HPV positivity between women with premature rupture of the membranes and women with a normal pregnancy(χ^(2)=0.67,P>0.05).High-risk and low-risk HPV differed significantly in pregnant women with premature rupture of the membranes(χ^(2)=29.17,P<0.01).High-risk and low-risk HPV differed significantly in women with a normal pregnancy(χ^(2)=50.82,P<0.01).Postpartum fetal membrane and amnionic fluid samples from 200 pregnant women with premature rupture of the membranes tested positive for HPV at a rate of 14%,including 24 women(85.71%)with high-risk HPV and 4 women(14.29%)with low-risk HPV.Cervical secretions from 200 normal pregnant women tested positive for HPV at a rate of 3%,and all 6 women were infected with high-risk HPV.Positivity for HPV in fetal membrane tissue and amniotic fluid differed significantly between pregnant women with premature rupture of the

关 键 词：人乳头瘤病毒HPV 胎膜早破 宫内感染 

分 类 号：R373[医药卫生—病原生物学]