基于HPLC的‘关山’樱花嫩红素成分——异樱花苷和樱花亭的定量分析  

Quantitative analysis of isosakuranin, sakuranetin in Prunus lannesiana‘Alborosea’ based on HPLC

作　　者：付涛[1] 黄增芳 施源 徐金华 沈雨铠 王志龙[1] Fu Tao;Huang Zengfang;Shi Yuan;Xu Jinhua;Shen Yukai;Wang Zhilong(Ningbo Key Laboratory of Landscape Plant Development/Ningbo City College of Vocational Technology,Ningbo 315100,China;Ningbo Forest Farm,Ningbo 315100,China;Shengzhou Landscape&Landscaping Company Limited,Shaoxing,312400,China)

机构地区：[1]宁波城市职业技术学院/浙江园林绿化技术协同创新中心,浙江宁波315100 [2]宁波市林场,浙江宁波315100 [3]嵊州市景观园林绿化有限公司,浙江绍兴312400

出　　处：《江苏林业科技》2022年第1期54-57,共4页Journal of Jiangsu Forestry Science & Technology

基　　金：浙江省教育厅一般科研项目“樱花嫩红素的高效提取工艺研究”(ZWT20098);大学生科技创新资助项目“樱花嫩红素的超声波辅助提取工艺探究及其应用”。

摘　　要：为了准确测定樱花中有关嫩红素主要成分的含量,该研究以我国南方主栽樱花品种‘关山’为试验材料,利用HPLC法对其花瓣干品内的嫩红素——异樱花苷和樱花亭进行定量分析。结果表明,在最适色谱条件下,异樱花苷的回归方程为Y=554.895353X+1567.125008(R^(2)=0.99980996),樱花亭的回归方程为Y=221.578696X+308.044811(R^(2)=0.99246515)。试验的精密度、稳定性、重复性和加样回收率结果均为良好。最终样品进样得到‘关山’樱花干品中异樱花苷平均含量为539.97μg/g,但未检测到樱花亭含量。该研究结果为提高我国樱花资源的应用范围及我国樱花资源的开发、利用,提供一定的依据。To accurately determine the content of main components of emodin in cherry blossom, this study used Prunus lannesiana ‘Alborosea’ planted for main variety in South China as experimental material, isosakuranin and sakuranetin were quantitatively analyzed by HPLC. Results showed that under the optimum chromatographic conditions, the regression equation of isosakuranin was concluded as Y=554.895 353X+1 567.125 008(R^(2)=0.999 809 96), and that of sakuranetin as Y=221.578 696X+308.044 811(R^(2)=0.992 465 15). The precision, stability, repeatability and sample recovery of the experiment were satisfactory. The average content of isosakuranin was determined as 539.97 μg/g in P. lannesiana ‘Alborosea’ dry petal product but sakuranetin was not detected. This results could improve the application scope of cherry resources and provide a basis for the development and utilization of cherry resources in China.

关 键 词：‘关山’樱花 嫩红素 异樱花苷 樱花亭 HPLC 

分 类 号：O652.63[理学—分析化学] Q946.82[理学—化学]